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Study on the Signal Responsive Chemical Release Systems

信号响应化学释放系统的研究

基本信息

批准号：
07458260
负责人：
KAETSU Isao
金额：
$ 1.02万
依托单位：
KINKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

The studies have been carried out for the purpose of constructions of unit systems which sense the various signal inputs from the environment and release the substances in feedback responses to the signals, and also for the purpose of integration of various unit systems selectively to transfer the environmental signals and the substance releases as the whole system. First, a combination system of a ISFET (Ion Sensitive Field Effect Transistor) biochip sencor with the immobilized enzymes and a substance release device was constructed. It proved the drug release function in response to additions of substrate compound. Then, a construction of more compact signal responsive substance release system was investigated using fine porous polymeric membranes to form molecular sensor-gate layrs in the porous structure. Namely, micro-porous membranes such as polyethylene terephthalate and polycarbonate was filled with polyelectrolyte gate layrs by a polymerization method, in which various molecules such as enzyme, photochromic compound, electro-conductive compound, were immobilized. The porous membranes with those gates proved various responsiveness such as pH-responsive, substrate-responsive, photo-responsive and electro-responsive release functions, respectively. Furthermore, some different kinds of unit systems were combined in series and parallel into an integrated whole system. It was proved clearly that the original signal input in the initial system, could be transferred selectively into the final end system, through the intermediate signal responsive substance releases.

进行这些研究的目的是为了构建感测来自环境的各种信号输入并在对信号的反馈响应中释放物质的单元系统，并且还为了选择性地整合各种单元系统以将环境信号和物质释放作为整个系统来传递的目的。首先，构建了ISFET（离子敏感场效应晶体管）生物芯片传感器与固定化酶和物质释放装置的组合系统。它证明了响应于底物化合物的添加的药物释放功能。然后，一个更紧凑的信号响应物质释放系统的结构进行了研究，使用精细的多孔聚合物膜，以形成分子传感器-门层的多孔结构。也就是说，通过聚合方法，在微孔膜如聚对苯二甲酸乙二醇酯和聚碳酸酯中填充生物膜层，其中固定了各种分子如酶、光致变色化合物、导电化合物。具有这些门的多孔膜证明了各种响应性，如pH响应，底物响应，光响应和电响应释放功能，分别。将不同类型的单元系统串并联组合成一个完整的系统。实验结果表明，在初始系统中输入的原始信号，可以通过中间信号响应物质的释放，选择性地传递到最终系统中。