Development of High Sensible Micuro-Glucose Sensor for Long-Term Implantation in Subcutaneous Tissue

开发用于皮下组织长期植入的高灵敏度微葡萄糖传感器

• 批准号: 07555560
• 负责人: SUDOH Masao
• 金额: $ 2.11万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07555560/
• 关键词: Glucose Sensor; Linearity Relationship; Design of Membranes; Modulus M_G; Oxygen Tension; Subcutaneous Tissue; 荷電膜; 交換吸着; 血糖値; 皮下組織; マイクロダイアリシス; 透析膜; 生体適合性; タンパク質吸着

Micro-glucose sensor for sensing glucose level in subcutaneous tissue was prepared and tested in vitro and in vivo. Glucose sensor consists of four membranes, which are a glucose sensible membrane, a semi-permeable membrane, a permselective membrane and a biocompatible membrane. The objective of this paper is to clarify a design of the sensor membranes and to develop a high sensible micro-glucose sensor for implantation in subcutaneous tissue. A theoretical analysis was made by considering laminated membranes of a glucose membrane and a permselective. When a sensor has high permselectivity of Oxygen to glucose, the larger value of M_G improved the more linearity relationship between output current and glucose concentration was. In order to monitor glucose level under low oxygen tension of 5% like subcutaneous tissue, the sensor was dip coated with 3% polyurethane solution in 98% tetrahydrofuran-2% dimethylformamide. The calibration curve of in uitro showed a linear relationship with glucose level over 500 mg・dL^<-1>, when sensor was covered with glucose sensible membrane which M_G was 0.8 and polyurethane membrane as permselective membrane. Output of the sensor was about 10nA/100mg・dL^<-1>-glucose. Glucose level in subcutaneous tissue was monitored continuously up to 7 hours by the sensor covered with Nafion as a biocompatible membrane.

研制了一种用于检测皮下组织葡萄糖浓度的微型葡萄糖传感器，并进行了体内外实验。葡萄糖传感器由葡萄糖敏感膜、半渗透膜、选择性渗透膜和生物相容性膜组成。本文的目的是阐明传感器膜的设计，并开发一种高灵敏度的微型葡萄糖传感器植入皮下组织。通过考虑葡萄糖膜和选择性渗透膜的层压膜进行了理论分析。当传感器对葡萄糖的选择透过性高时，M_G值越大，输出电流与葡萄糖浓度的线性关系越好。为了像皮下组织一样在5%的低氧张力下监测葡萄糖水平，传感器浸涂有在98%四氢呋喃-2%二甲基甲酰胺中的3%聚氨酯溶液。以<-1>葡萄糖敏感膜（M_G = 0.8）和聚氨酯膜为选择透过膜，在500 mg·dL ~（-1）以上的葡萄糖浓度范围内，其校准曲线呈线性关系。传感器的输出约为10 nA/100 mg·dL^<-1>-葡萄糖。通过覆盖有Nafion作为生物相容性膜的传感器连续监测皮下组织中的葡萄糖水平长达7小时。

项目成果

須藤雅夫: "メディエーター型簡易血糖値モニターの性能試験" 化学工学シンポジウムシリーズ. 58巻. 42-45 (1997)

须藤正男：“介体型简易血糖值监测仪的性能测试”化学工程研讨会系列第58卷42-45（1997年）。

M.Sudoh: "Non-homogeneous Intramembrane Structure and Transport Properties of ion-Exchange Membrane" J.ION EXCHANGE. 8. 202-213 (1997)

M.Sudoh：“离子交换膜的非均质膜内结构和传输特性”J.ION EXCHANGE。

須藤雅夫: "低温プラズマ重合によるアクリル酸及びフッ素系スルフォン酸荷電膜の合成と輸送" 繊維学会誌. 52. 110-115 (1996)

Masao Sudo：“通过低温等离子体聚合的丙烯酸和氟化磺酸的带电膜的合成和传输”日本纺织科学技术学院学报52. 110-115（1996）。

須藤雅夫: "Effect of diffusion and reaction within enzyme membrane on output current of a diaposable monitor of blood glucose" Proceedings of ICOM-96. 780-781 (1996)

Masao Sudo：“酶膜内扩散和反应对可降解血糖监测仪输出电流的影响”，ICOM-96 论文集（1996 年）。

須藤雅夫: "イオン交換膜の不均質膜内構造と輸送特性" J.ION EXCHANGE. 8巻. 202-213 (1997)

Masao Sudo：“离子交换膜的异质膜内结构和传输特性”J.ION EXCHANGE Vol. 8. 202-213 (1997)