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Chromosome mapping of genes in mammals and birds and its applcation for animal resource sciences

哺乳动物和鸟类基因染色体图谱及其在动物资源科学中的应用

基本信息

批准号：
07556114
负责人：
TAKAHASHI Shin-ichiro
金额：
$ 4.1万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556114/
关键词：
GARS AIRS GART fused gene evolution human chromosome 21 プリン生合成酵素遺伝子霊長類における進化

项目摘要

Cloning of a human cDNA encoding the trifunctional glycinamide ribonucleotide synthetase (GARS) -aminoimidazole ribonucleotide synthetase (AIRS) -glycinamide ribonucleotide transformylase (GART) enzyme by functional complementation in E.coli and in yeast has been reported previously. In this study, we demonstrates the isolation of a novel cDNA clone encoding only the GARS domain from a human fetal brain cDNA library using the 5' region of the human GARS-AIRS-GART cDNA as a probe. Analysis of the nucleotide sequence reveals that the coding region of this cDNA is identical to that of the GARS domain of the GARS-AIRS-GART gene cDNA.Analysis of the expression patterns of the two transcripts in normal human brains of various ages demonstrates that each is transcribed at different overall levels depending upon age. We concluded that the human GARS-AIRS-GART gene produces the trifunctional enzyme and the human gene encoding only the GARS domain produced the enzyme showing the activity of only GARS.In addition, the connecting region of GARS and AIRS in the GARS-AIRS-GART gene were completely preserved between the primates. On the contrary, in the 3' untranslate region of the GARS gene we observed a few replacements, inserts as well as deletion of nucleic acids between human and other primates. In conclusion, these results strongly suggested that the GARS-AIRS-GART gene is fused from the independent GARS,AIRS,GART genes evolutionally. Using the chromosome mapping techniques, we continuously intend to analyze other important genes for animal resource sciences.

先前已经报道了在大肠杆菌和酵母中通过功能互补克隆编码三功能甘氨酰胺核糖核苷酸合成酶（加尔斯）-氨基咪唑核糖核苷酸合成酶（AIRS）-甘氨酰胺核糖核苷酸转化酶（GART）的人cDNA。本研究以人GARS-AIRS-GART cDNA的5'端序列为探针，从人胎脑cDNA文库中克隆了一个仅编码加尔斯结构域的cDNA克隆。核苷酸序列的分析表明，该cDNA的编码区与GARS-AIRS-GART基因cDNA的加尔斯结构域的编码区相同。对不同年龄的正常人脑中这两种转录本的表达模式的分析表明，根据年龄，每种转录本在不同的总体水平上转录。结果表明，人GARS-AIRS-GART基因产生三功能酶，而人仅编码加尔斯结构域的基因产生的酶仅具有加尔斯的活性，并且GARS-AIRS-GART基因中的加尔斯和AIRS的连接区在灵长类动物中完整保存。相反，在加尔斯基因的3'非翻译区，我们观察到一些替换，插入以及在人类和其他灵长类动物之间的核酸缺失。这些结果表明，GARS-AIRS-GART基因是由独立的加尔斯、AIRS、GART基因在进化上融合而成的。利用染色体定位技术，我们将继续对动物资源科学的其他重要基因进行分析。