The roles of growth factors and cytokine in retinal neovascularization.

生长因子和细胞因子在视网膜新生血管形成中的作用。

• 批准号: 07557263
• 负责人: SAITO Yoshihiro
• 金额: $ 1.86万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557263/
• 关键词: neovascularization; Agerelated macular degeneration; thalidomide; laser; retina; bFGF; Diabetes; Retinopathy of prematurity; 加齢黄斑変性; 脈絡膜; 光化学療法; フルオレスチンナトリウム

We developed new animal models of ocular neovascularization. A rat model of subretinal neovascularization was made by a b-FGF containing pellet implanted between sensory retinal and retinal pigment epithelium. The subretinal neovascularization was very similar to human age-related macular degeneration histologically and histopathologically. Another model was preretinal neovascularization in rats, which was made by artificial retinal vein occlusion with photodynamic (Ar laser and fluorescein-Na) thrombosis in rat. This was the first rat model of preretinal neovascularization.Thalidomide is a well known teratogen in human. The drug which had an inhibitory effect in angiogenesis for rabbit corneal neovascularization is the only antiangiogenesis agent that is currently ready for human efficacy trials. We investigated the inhibitory effect of thalidomide using preretinal neovascularization model. Though there was no significant difference, it tended to have an inhibitory effect on retinal neovascularization in rats.We investigated the roles of growth factors and cytokines in pre- and sub- retinal neovascularization using rat models. We found a-FGF,b-FGF and VEGF had significant role in the development of above neovascularizations.We also attempted to improve the surgical result or clinical knowledge of diseases which develop the preretinal and/or subretinal neovascularization : retinopathy of prematurity, diabetes, age-related macular degeneration.

我们建立了新的眼部新生血管动物模型。将含有b-成纤维细胞生长因子的微球植入视网膜感觉上皮和视网膜色素上皮之间，建立大鼠视网膜下新生血管模型。视网膜下新生血管在组织学和组织病理学上与人类老年性黄斑变性非常相似。另一种是大鼠视网膜前新生血管模型，采用人工视网膜静脉阻塞加光动力(Ar激光和荧光素钠)血栓形成的方法。这是第一个视网膜前新生血管的大鼠模型。沙利度胺是一种众所周知的人类致畸物质。该药物对兔角膜新生血管具有抑制血管生成的作用，是目前唯一准备进行人类疗效试验的抗血管生成药物。我们用视网膜前新生血管模型研究了沙利度胺的抑制作用。虽然没有显著差异，但对大鼠视网膜新生血管有抑制作用。我们利用大鼠模型研究了生长因子和细胞因子在视网膜前和视网膜下新生血管中的作用。我们发现a-FGF、b-FGF和VEGF在上述新生血管的形成过程中起着重要的作用。我们还试图提高对视网膜前和/或视网膜下新生血管疾病的手术疗效或临床知识：早产儿视网膜病变、糖尿病、老年性黄斑变性。

项目成果

Tsujikaw M.Saito et al.: "Secondary vitrecfony for the treatment of macular〜" Ophthalmic Surgery and lasers. (印刷中).

Tsujikaw M.Saito 等人：“治疗黄斑的继发性玻璃体切除术 ~”眼科手术和激光（出版中）。

Sawa M,Kamei M,Tsujikawa M,Ohji M,Saito, Y,et al.: "Outer blood-retinal barrior reconstruction after surgical removal of choroidal neovascularization." ARVO (May 11 16,1997).

Sawa M，Kamei M，Tsujikawa M，Ohji M，Saito，Y，et al.：“手术切除脉络膜新生血管后的外血视网膜屏障重建。”

Saito, Y,et al.: "A lens change and related symptoms during rapid tightening of metabolic control in diabetes." ARVO (May 11-16,1997).

Saito, Y 等人：“快速加强糖尿病代谢控制过程中的晶状体变化和相关症状。”

Ohji M,Saito Y,et al.: "Removal of subretinal choroidal neovascular membranes without gas tamponade." ARVO (May 11-16,1997).

Ohji M、Saito Y 等人：“无需气体填塞即可去除视网膜下脉络膜新生血管膜。”

Nakabayashi M,Fujikado T,Tsujikawa M,Ohji M,Saito Y,et al.: "Fixation patterns of macular hole after successful vitreous surgery." ARVO (May 11-16,1997).

Nakabayashi M，Fujikado T，Tsujikawa M，Ohji M，Saito Y，et al.：“成功玻璃体手术后黄斑裂孔的固定模式。”