The roles of growth factors and cytokine in retinal neovascularization.

我们开发了新的眼部新生血管动物模型。通过在感觉视网膜和视网膜色素上皮之间植入含有b-FGF的小球来制备视网膜下新生血管形成的大鼠模型。视网膜下新生血管在组织学和组织病理学上与人类年龄相关性黄斑变性非常相似。另一种模型为大鼠视网膜前新生血管模型，采用氩激光和荧光素钠联合光动力学血栓栓塞法制备大鼠视网膜前新生血管模型。这是第一个大鼠视网膜前新生血管模型。该药物对兔角膜新生血管的血管生成具有抑制作用，是目前唯一准备进行人体疗效试验的抗血管生成剂。我们使用视网膜前新生血管模型研究沙利度胺的抑制作用。本实验采用大鼠视网膜新生血管模型，研究生长因子和细胞因子在视网膜新生血管形成中的作用。本研究发现a-FGF、b-FGF和VEGF在上述新生血管的形成中起重要作用，并试图提高对早产儿视网膜病变、糖尿病、老年性黄斑变性等发生视网膜前和/或视网膜下新生血管的疾病的手术效果或临床认识。

We developed new animal models of ocular neovascularization. A rat model of subretinal neovascularization was made by a b-FGF containing pellet implanted between sensory retinal and retinal pigment epithelium. The subretinal neovascularization was very similar to human age-related macular degeneration histologically and histopathologically. Another model was preretinal neovascularization in rats, which was made by artificial retinal vein occlusion with photodynamic (Ar laser and fluorescein-Na) thrombosis in rat. This was the first rat model of preretinal neovascularization.Thalidomide is a well known teratogen in human. The drug which had an inhibitory effect in angiogenesis for rabbit corneal neovascularization is the only antiangiogenesis agent that is currently ready for human efficacy trials. We investigated the inhibitory effect of thalidomide using preretinal neovascularization model. Though there was no significant difference, it tended to have an inhibitory effect on retinal neovascularization in rats.We investigated the roles of growth factors and cytokines in pre- and sub- retinal neovascularization using rat models. We found a-FGF,b-FGF and VEGF had significant role in the development of above neovascularizations.We also attempted to improve the surgical result or clinical knowledge of diseases which develop the preretinal and/or subretinal neovascularization : retinopathy of prematurity, diabetes, age-related macular degeneration.