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Analysis of hemodynamics in intramyocardial microcirculation using needle-probe fluorescent intravital microscope

针探针荧光活体显微镜分析心肌内微循环的血流动力学

基本信息

批准号：
07558133
负责人：
MORITA Koichi
金额：
$ 3.33万
依托单位：
Kawasaki Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

To visualize the subendocardial arteriolar blood velocity, we have developed a high-speed CCD microscope. The vascular images, focused on the special CCD through a needle-probe, were stored on a digital memory every 5 ms (200images/s). A size of visual field is 1*0.8mm^2, and a spatial resolution is less than 4mum. The blood flow velocities in the microvessels were visualized with optically shinning microspheres (MS) as optical marker. The optical marker (5-15 mumphi) was prepared by polymerization of stirene monomer with homogeneously distributed fine particles of heavy elements (0.2 mumphi). Velocities of MS in the subepicardial and subendocardial arteriole were calculated by the movement distance of MS every 5 ms. To visualize flow on the monitor, the MS was injected into the left anterior descending coronary artery. The MS images of first-pass were recorded on a video-cassette recorder. Positions of MS in the microvessels were measured using a computer system. Velocities of MS in the subendocardial arteriole were calculated by frame-to-frame analysis from the movement distance of MS.In endocardial arterioles, fast forward flow was observed during diastole, while there was retrograde flow with two peaks during systole. In epicardial arterioles, fast forward flow was observed during diastole, similar to the subendocardial blood flow. However, a plenty of forward flow was also observed in systolic phase. The novel achievement in this study is the direct observation of subendocardial blood flow in arterioles during a cardiac cycles at highly temporal resolution.

为了观察心内膜下小动脉血流速度，我们研制了高速CCD显微镜。血管图像通过针状探针聚焦在专用CCD上，每5 ms（200张/s）存储在数字存储器中。视野尺寸为1*0.8mm^2，空间分辨率小于4mm。用光学发光微球（MS）作为光学标记，观察微血管血流速度。用均匀分布的重元素细颗粒（0.2 mumphi）聚合乙烯单体制备光学标记物（5-15 mumphi）。以MS每5ms的运动距离计算MS在心外膜下和心内膜下小动脉的运动速度。为了在监视器上显示血流，将MS注入左冠状动脉前降支。第一次通过的质谱图像记录在盒式录像机上。用计算机系统测量微血管中质谱的位置。根据MS的运动距离，通过帧对帧的分析计算心内膜下小动脉MS的速度。心内膜小动脉舒张期为快速向前血流，收缩期为两峰逆行血流。在心外膜小动脉中，舒张期观察到快速向前流动，与心内膜下血流相似。然而，在收缩期也观察到大量的正向血流。这项研究的新成果是在心脏周期中以高时间分辨率直接观察心内膜下小动脉的血流。