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Difperent sensitiorty UVB radiation of rice cultioors based on UVB-caused CPDs Frequencies

基于 UVB 引起的 CPD 频率的水稻栽培不同敏感性 UVB 辐射

基本信息

批准号：
07558202
负责人：
KUMAGAI Tadashi
金额：
$ 0.58万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558202/
关键词：
DNA pyrimsdine dimer photo-reposr excision repasr ultraosdet B high nolecular DNA danage analyzer rice

项目摘要

We previously fornd that UVB reduced tiller number and biomass, and the amounts of soluble protein, Rubisco and so on in leaves of rice. There were cultivar differences in the sensitivity to the inhibitory effects of UVB among indica, javanica and japonica rice cultivars. Among Japanese rice cultivars, Sasanishiki exhibited more resistance to UVB,while Norin 1 was less resistant. In this work, we examined if differences in cyclobutyl pyrimidine dimer frequencies (CPD^S) caused by UVB were involved in UV-sensitive and UV-resistant cvs. Norin 1 and Sasanishiki. We also investigated the changes in UVB-caused CPDs in leaf developed at the different stages in rice grown in visible radiation without UVB.We improved an alkaline agarose gel electrophoresis using unidirectional pulse field gel electrophoresis devised by Sutherland et al.(1987). Using this apparatus, we first found that Norin 1 was more deficient in the repair ability of CPDs by photoreactivation and excision than Sasanishiki. The leaf fully expanded at the different stages was detache from plant, and exposed to UVB (9KJ/m2) : the highest CPDs were shown to be produced in the 2,3 and 4th fully expanded leaf. Furthemore, the changes of UVB-caused CPDs were examined in the different aged-leaf of the 6th leaf from leaf emergence through completion of expansion.The leaf grown for 1,4,7 and 15 days after leaf emergence was exposed to the above mentioned dose of UVB.The 6th leaf was fully expanded 7 days after emergence. The highest CPDs were found to be produced in the 4 days-grown leaf. It was thus indicated that the sensitivity to UVB caused CPDs dramatically changed in the progress of growth and leaf age.

我们已经发现UVB降低了水稻分蘖数和生物量，降低了水稻叶片中可溶性蛋白、Rubisco等的含量。籼稻、爪哇稻和粳稻品种对UVB抑制效应的敏感性存在品种差异。在日本品种中，佐三石木对UVB的抗性较强，而野林1号的抗性较弱。在这项工作中，我们研究了UVB引起的环丁基嘧啶二聚体频率（CPD^S）的差异是否与紫外线敏感和抗紫外线cvs有关。Norin 1和Sasanishiki。我们还研究了在无UVB可见辐射条件下生长的水稻叶片不同时期UVB引起的CPDs的变化。我们使用Sutherland等人（1987）设计的单向脉冲场凝胶电泳改进了碱性琼脂糖凝胶电泳。利用该装置，我们首次发现Norin 1比Sasanishiki更缺乏光活化和切除CPDs的修复能力。将不同时期完全展开的叶片与植株分离，暴露于UVB （9KJ/m2）下，CPDs在完全展开的第2、3和4期叶片中产生最高。此外，研究了uvb引起的CPDs在第6叶的不同年龄叶片从叶片出苗到完成膨化的变化。叶片出芽后生长1、4、7和15 d的叶片分别暴露于上述剂量的UVB。第6叶在羽化后7 d完全展开。生长4 d叶片的CPDs最高。由此可见，随着生长和叶龄的变化，对UVB的敏感性引起了CPDs的显著变化。