Measurement System of Endothelium-Derived Nitric Oxide Release Induced by Blood Flow

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We have attempted to directly measure Nitric Oxide (NO) concentration in the arterial vascular wall, which is induced by blood flow (wall shear stress) using an NO sensing electrode. The NO microelectrode (100 mum in dimeter) was inserted into the vascular media of isolated canine femoral arteries under observation of a light microscope using a micro manipulator. The vessel was then perfused with a Krebs-Henseleit solution, and the flow-related change in NO production was measured by altering perfusion rate. NO concentration in the vascular media increased linearly with increasing perfusion rate (n=7, r^2=0.90-0.99, p<0.02). NO production was increased by perfusing a solution containing L-arginine and was attenuated by N^G--monomethyl-L-arginine (L-NMMA) and N^G-nitro-L-arginine (L-NA), indicating that the NO microelectrode measures NO selectively. We thus confirmed that the concentration of NO in the arterial vascular media changes with changing perfusion rate.