Evaluation of the three dimendional microvascular structure and physiological function of renal glomerulus using superphoton derived X-ray micro focus CT

超光子X射线微焦点CT评价肾小球三维微血管结构及生理功能

• 批准号: 13680959
• 负责人: OGASAWARA Yasuo
• 金额: $ 2.3万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680959/
• 关键词: superphoton; X- ray micro focus CT; glomerulus; three dimentional structure; microcirculation; 三次元血管構造

We aimed at visualizing thee dimensional (3D) glomerular micro- structures using a superphoton derived X- ray micro focus CT (Mct) to accurately measure glomerular volumes throughout a cortex and quantitatively evakuate global heterogeneity of the volume- distribution in a rat with spontaneous diabetes mellitus (DM). Using the Otsuka Long Evans Tokushima Fatty (OLETF) rats as rats of early stage DM (28 weeks, n=5) and control rats (Long Evans Tokushima Fatty; LETO, n=5) of the same age, the vessels of the left kidney was filled up with contrast media (BaSO_4 + India ink + 8%gelatin). A sampled renal column (3-4 mm) was observed using two Mct system; 8GeV- superphoton Mct for the highest image quality (3-6 mm in resolution) and commercially available Mct (10 mm in resolution) for data accumulation. The glomerular volumes more than 400 per sample were computed by coefficient variation (CV). By stereomicroscopic observation, we confirmed complete- filling of the vessels with the contrast media. The glomeruli in OLETF were characterized by irregular stereostractures, whereas those in LETO were characterized by regular glomerular structure. The CV of glomerular volume was significantly larger in OLETF than LETO (p<0.01). In addition, absolute glomerular volume was larger in OLETF than LETO (p<0.005). The normalized glomerular volume was, however, comparable (NS), indicating glomerular enlargement in early DM may associate with increase in body mass. Both Mct provided similar results. Highly heterogeneous glomerular volume- distribution in DM rat was quantitatively demonstrated using our developed 3D glomerular micro- structural visualizing method. This technique provides a new aspect for the evaluation of global heterogeneity in glomerular remodeling under the chronic renal dysfunction, which may provide more sensitive insight into early complications of diabetic nephropathy

我们旨在利用超光子衍生X射线微聚焦CT （Mct）可视化三维肾小球微观结构，以准确测量整个皮质的肾小球体积，并定量评估自发性糖尿病（DM）大鼠体积分布的全球异质性。将大鼠（OLETF）作为早期DM大鼠（28周龄，n=5）和对照大鼠（LETO, n=5），用对比剂（BaSO_4 +印度墨水+ 8%明胶）填充左肾血管。采用双Mct系统观察肾柱（3-4 mm）；8GeV-用于最高图像质量（3-6毫米分辨率）的超光子Mct和用于数据积累的商用Mct（10毫米分辨率）。通过变异系数（CV）计算每个样本超过400个的肾小球体积。通过立体显微镜观察，我们证实造影剂完全填充血管。OLETF组肾小球呈不规则立体结构，而LETO组肾小球呈规则结构。OLETF组肾小球体积CV显著高于LETO组（p<0.01）。此外，OLETF组的肾小球绝对体积大于LETO组（p<0.005）。然而，正常肾小球体积相当（NS），表明早期糖尿病肾小球增大可能与体重增加有关。两项Mct都提供了类似的结果。我们开发的三维肾小球微观结构可视化方法定量显示了糖尿病大鼠肾小球体积分布的高度异质性。该技术为评估慢性肾功能障碍下肾小球重构的全球异质性提供了一个新的视角，可能为糖尿病肾病的早期并发症提供更敏感的见解

项目成果

Tokunori Yamamoto: "In vivo visualization of angiotensin II-and tubul oglomerul ar feedback-medi ated renal vasoconstriction."Kidney International. 60. 364-369 (2001)

Tokunori Yamamoto：“血管紧张素 II 和肾小管反馈介导的肾血管收缩的体内可视化。”肾脏国际。

Yasuo Ogasawara: "Analysis of three dimensional architecture of renal microcirculation"Spring- 8 User Experiment. Report. 125 (2002)

小笠原康夫：《肾脏微循环三维架构分析》Spring-8 用户实验。

Yasuo Ogasawara: "Global heterogeneity of glomerular volume-distribution evaluated by three-dimensional analysis using synchrotron radiation X-ray microCT"SPring-8 Research Frontiers 2000/2001. 27-28 (2002)

Yasuo Ogasawara：“使用同步辐射X射线显微CT通过三维分析评估肾小球体积分布的整体异质性”SPring-8研究前沿2000/2001。

Fumihiko Kajiya: "Tissue Engineering for Therapeutic Use 5"Elsevier Science B.V.. 12 (2001)

Fumihiko Kajiya：“治疗用途的组织工程 5”Elsevier Science B.V.. 12 (2001)

Keiji Umetani: "Observation of Cardiac Microcirculation Using High Speed Microangiography System"Spring-8 User Experiment. Report. 161 (2002)

Keiji Umetani：“使用高速微血管造影系统观察心脏微循环”Spring-8 用户实验。