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Liquid Chromatograph-Catalytic Analysis as a Automatic and Fractional Determination Method with Extremely High Sensitivity

液相色谱催化分析作为一种具有极高灵敏度的自动分级测定方法

基本信息

批准号：
07640802
负责人：
HORI Toshitaka
金额：
$ 1.47万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07640802/
关键词：
Liquid Chromatography Catalytic Analysis Automatic determination

项目摘要

1 Automatic and fractional determination of vanadium (IV and V) and iron (II and III) by liquid chromatograph-catalytic analysisVanadium was determined sensitively by air-segmented continuous flow analysis (ASCFA) based on a catalytic reaction with potassium bromate and Bindschedler's green leuco base. Vanadium (IV and V) were separated each other by using the Tosoh model TSK gel IC-Cation as a separation column, and the mixture (pH 3.0) of 0.2 mM ethylenediamine and 1.0 mM tartaric acid as a eluate. By connecting these two methods, vanadium (IV and V) were determined automatically and fractionally. Determination range by the present method was 5 - 300 nM for each vanadium speciese.Iron was also determined by the above catalytic reaction. However, we have not succeed to separate vanadium and iron by liquid chromatography. We have been investigating their separation.2 Automatic and fractional determination of cobalt, manganese and iron by liquid chromatograph - catalytic analysisCobalt, manganese and iron were separated one another by using Shimadzu model Shim-pack IC-C1 gel as a separation column, and 0.3 M lactic acid as a eluate. These elements were determined by ASCFA based on a catalytic reaction with hydrogen peroxide and tiron. Now, we have been trying to connect these two methods.3 Elemental composition of waters and deposits collected along the acid river SukawaRedox potential of VO_2^+/VO^<2+> suggests that vanadium (IV) dissolves more stably in acid solution. We investigated preliminarily the elemental composition of waters and deposits collected along the acid river Sukawa, Yamagata Pref., in order to apply our liquid chromatograph-catalytic analysis for vanadium (IV and V) to natural acid water samples. Total concentration of vanadium (IV and V) was very high and ranged from 2 to 5 muM.

1液相色谱-催化分析法自动分级测定钒（IV和V）和铁（II和III）基于溴酸钾和Bindschedler的绿色隐色碱的催化反应，采用空气分段连续流动分析法（ASCFA）灵敏地测定钒。通过使用Tosoh型号TSK凝胶IC-Cation作为分离柱，和0.2mM乙二胺和1.0mM酒石酸的混合物（pH 3.0）作为洗脱剂，将钒（IV和V）彼此分离。将这两种方法有机地结合起来，实现了钒（IV）和钒（V）的自动分级测定。测定范围为5 - 300 nM，铁也可通过上述催化反应测定。然而，我们还没有成功地用液相色谱法分离钒和铁。2.液相色谱-催化分析法自动分次测定钴、锰、铁采用岛津Shim-pack IC-C1凝胶为分离柱，以0.3M乳酸为缓冲液，钴、锰、铁相互分离。这些元素是由ASCFA基于与过氧化氢和Tiron的催化反应测定的。现在，我们一直在尝试将这两种方法联系起来。3沿着酸性河流Sukawa收集的沃茨水和沉积物的元素组成VO_2^+/VO^<2+>的氧化还原电位表明，钒（IV）在酸性溶液中溶解得更稳定。我们对山形县须川酸性河流沿岸沿着采集的沃茨和沉积物的元素组成进行了初步调查，目的：将我们的液相色谱-催化分析法应用于天然酸性水样中钒（IV和V）的测定。钒（IV和V）的总浓度非常高，范围为2至5 μ M。