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Origin and Evolution of Neural Crest Cells in Chordates

脊索动物神经嵴细胞的起源和进化

基本信息

批准号：
07640883
负责人：
TSUNEKI Kazuhiko
金额：
$ 1.41万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07640883/
关键词：
neural crest cyclostome teleost extracellular matrix immunohistochemistry 神経冠細胞

项目摘要

1.The neural crest is a characteristic tissue of vertebrate embryos. Neural crest cells appear on the dorsal side of the neural tube, migrate to various regions of the body, and differentiate into a variety of cells. Thus, the neural crest participates in forming the vertebrate body organization. At first, migration and colonization patterns of neural crest cells were studied in the most primitive vertebrate, the lamprey, using a monoclonal antibody, HNK-1, which stains migratory neural crest cells.(1) In the trunk of lamprey embryos, neural crest cells migrate in three routes ; the dorsolateral route between epidermis and somite, the ventral route between somite and neural tube, and the dorsal route towards the dorsal fin rudiment. In the ventral route, these cells do not migrate ventrally beyond the notochord. This may be related to the absence of sympathetic chains in lampreys. The neural crest cells in the fin rudiment may differentiate into mesenchymal cells and form part of the d … More orsal fin.(2) In lamprey embryos, sensory "doral cells" exist in the neural tube and they are stained with HNK-1. HNK-1-immunoreactive nerve fibers are also detected sub-epidermally and at least some of the fibers originate from dorsal cells. Sensory spinal ganglia are very small in lampreys. Dorsal cells may differentiate from "non-migratory neural crest cells". These observations are relevant to the origin of neural crest and/or placode.2.The migration and colonization patterns of neural crest cells were also studied in the swordtail embryo, using HNK-1 and some antibodies against extracellular matrix molecules.(1) In the trunk of swordtail embryos, three migration routes of neural crest cells are recognized, as in lamprey embryos. Neural crest cells in the ventral route colonize the region below the notochord and differentiate into sympathetic ganglion cells.(2) Extracellular materials such as fibronectin and chondroitin sulfate appear and distribute immunohistochemically, as HNK-1 immunoreactive neural crest cells appear and migrate. Thus, these materials may be intimately related to neural crest cell migration in the teleost as in the higher vertebrate. Less

1.神经嵴是脊椎动物胚胎的特征性组织。神经嵴细胞出现在神经管的背侧，迁移到身体的各个区域，并分化成各种细胞。因此，神经嵴参与了脊椎动物身体组织的形成。首先，在最原始的脊椎动物七鳃鳗，使用单克隆抗体，HNK-1，染色迁移神经嵴细胞的神经嵴细胞的迁移和定居模式进行了研究。(1)在七鳃鳗胚胎的躯干中，神经嵴细胞有三条迁移路线：表皮与体节之间的背外侧路线、体节与神经管之间的腹侧路线和朝向背鳍雏形的背侧路线。在腹侧路线中，这些细胞不迁移腹侧超过脊索。这可能与七鳃鳗缺乏交感神经链有关。鳍原基中的神经嵴细胞可分化为间充质细胞，并形成神经嵴的一部分。 ...更多信息背鳍(2)在七鳃鳗胚胎中，感觉“背侧细胞”存在于神经管中，它们用HNK-1染色。HNK-1免疫反应阳性神经纤维也检测到表皮下，至少有一些纤维起源于背细胞。七鳃鳗的感觉脊神经节非常小。背细胞可以从“非迁移性神经嵴细胞”分化而来。这些观察结果与神经嵴和/或基板的起源有关。2.用HNK-1和抗细胞外基质分子的抗体研究了剑尾鱼胚胎神经嵴细胞的迁移和定居模式。(1)在剑尾鱼胚胎的躯干中，神经嵴细胞的三条迁移路线被确认，在七鳃鳗胚胎中。腹侧通路的神经嵴细胞在脊索下方的区域定居并分化成交感神经节细胞。(2)随着HNK-1免疫反应阳性神经嵴细胞的出现和迁移，细胞外物质如纤维连接蛋白和硫酸软骨素的出现和分布呈免疫化学变化。因此，这些材料可能与硬骨鱼类和高等脊椎动物的神经嵴细胞迁移密切相关。少