Elucidation of race-cultivar specificity in blast by using mutant rice plants.

使用突变水稻植物阐明稻瘟病中的种族品种特异性。

• 批准号: 07660058
• 负责人: ARASE Sakae
• 金额: $ 1.41万
• 依托单位: SHIMANE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660058/
• 关键词: rice blast; mutant rice; toxin; race; イネいもち病菌; 胞子発芽液

The specificity between fungal race-plant cultivars in blast was studied by using mutant rice plants. When mutant rice plant, cv.Sekiguchi-asahi, was inoculated with Magnaporthe grisea spores, the Sekiguchi lesion were light-dependently formed on the leaves, This unique lesion formation was suppressed by pre-treatment with photo- and protein-synthesic inhibitors, DCMU and cycloheximide. Magnaporthe grisea releases a toxin(s) into the spore germination fluid, which allow anon-pathogen, Alternaria alternata, to infect host plant tissues. The role of a toxin(s) on establishment of basic compatibility between fungus and plant was investigated by ultrastructural observation and morphometric measurement. The first toxin-induced changes in ultrastructure were the losses of mitochondrial matrix and the disruption of mitochondrial cristae 1hr after toxin exposure. However, frequency of modified mitochondria did not increase with time after toxin treatment. Other organelles were normal even in t … More he late stage of the toxin treatment. These phenomena were observed in both Sekiguchi-asahi and Asahi leaves regardless of light condition. When the effects of the toxin(s) on host plants (rice and barley) and non-host plant (finger millet) were examined, the first toxin-induced changes in rice and barley plants were the loss of the mitochondrial matrix and the disruption of mitochondrial cristae. These modifications in mitochonria were observed 1 hr after toxin exposure. However, the frequency of modified mitochondria did not increase with time after toxin treatment. Other organelles were normal even in a late stage of the toxin treatment. Such phenomena were observed in three sites (epidermal, mesophyll and phloem cells) of host plants, barley and rice. However, the effects of toxin(s) were not observed in leaves of non-host plant, finger millet. Further, mitochondrial modifications were observed in both epidermal cells of compatible and incompatible cultivars 12hr after inoculation of M.grisea spores. These results indicated that mitochondrial modification induced by the toxin(s) was an essential event for M.grisea to establish basic compatibility with host plants at species level. Less

以突变体水稻为材料，研究了稻瘟病病菌小种-品种间的特异性。用稻瘟病菌（Magnaporthe grisea）孢子接种突变体Sekiguchi-asahi，可在叶片上形成光依赖性的Sekiguchi病斑。光和蛋白质合成抑制剂DCMU和放线菌酮预处理可抑制这种独特的病斑形成。稻瘟病菌释放毒素到孢子萌发液中，这使得非病原体互隔交链孢（Alternaria alternata）感染宿主植物组织。通过超微结构观察和形态计量学测量，研究了一种毒素在真菌与植物建立基本亲和性中的作用。毒素暴露1h后，线粒体的超微结构首先发生变化，表现为线粒体基质的丢失和嵴的断裂。然而，修饰的线粒体的频率并没有随着毒素处理后的时间增加。其他细胞器正常，即使在t ...更多信息 毒素治疗的后期无论光照条件如何，在Sekiguchi Asahi和Asahi叶片中都观察到这些现象。当毒素对寄主植物（水稻和大麦）和非寄主植物（指粟）的影响进行了检查，在水稻和大麦植物中的第一个毒素诱导的变化是线粒体基质的损失和线粒体嵴的破坏。这些修改线粒体毒素暴露后1小时观察。然而，修饰的线粒体的频率并没有随着毒素处理后的时间增加。其他细胞器正常，即使在毒素治疗的后期。这种现象在寄主植物大麦和水稻的表皮、叶肉和韧皮部细胞中均有观察到。而在非寄主植物谷子叶片中未观察到毒素的作用。接种稻瘟病菌孢子12小时后，亲和和不亲和品种的表皮细胞线粒体均发生了变化。这些结果表明，毒素诱导的线粒体修饰是稻瘟病菌在种水平上与寄主植物建立基本亲和性的必要事件。少

项目成果

Arase et al.: "Molecular aspect of pathogenicity and host resistance Requirments for signal transduction" Mills,D.& Kunoh,H.(in press), (1996)

Arase 等人：“信号转导的致病性和宿主抗性要求的分子方面”Mills，D.

Isota, J., Kadowaki, Y.and Arase, S.: "Spontaneous occurrence of the Sekiguchi lesion on rice cv.Himenomochi." Ann.Phytopathol.Soc.Jpn.62 (2). 167-169 (1996)

Isota, J.、Kadowaki, Y. 和 Arase, S.：“水稻 cv.Himenomochi 上 Sekiguchi 病变的自发发生。”

Arase, S., Yoshiura, Y., Ozoe, Y., Honda, Y.and Nozu, M.: "Production of a phytoalexin, Sacranetin, from"Sekiguchi lesion"on rice cv.Sekiguchi-asahi." Ann.Phytopathol.Soc.Jpn.62 (4). 408-410 (1996)

Arase, S.、Yoshiura, Y.、Ozoe, Y.、Honda, Y. 和 Nozu, M.：“从水稻品种 Sekiguchi-asahi 上的‘Sekiguchi 病变’中生产植物抗毒素 Sacranetin。”

Arase, S., Honda, Y.and Nozu, M.: In Molecular aspect of pathogenicity and resistance : Requirement for signal transduction (Mills et al.eds.). APS press, Minnesota, 274 (1996)

Arase, S.、Honda, Y. 和 Nozu, M.：致病性和耐药性的分子方面：信号转导的要求（Mills 等人编辑）。

Uehara, T., Arase, S., Honda, Y., Park, P.and Nozu, M.: "Ultrastructural effects of Magnaporthe grisea toxin (s) on mitochondria of host and non-host plants." Ann.Phytopathol.Soc.Jpn.63 (2) (in press). (1997)

Uehara, T.、Arase, S.、Honda, Y.、Park, P. 和 Nozu, M.：“稻瘟病菌毒素对宿主和非宿主植物线粒体的超微结构影响”。