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Glucose Metabolism and Insulin Action in Sheep Exposed to Cold

暴露于寒冷的绵羊的葡萄糖代谢和胰岛素作用

基本信息

批准号：
07660368
负责人：
SANO Hiroaki
金额：
$ 1.34万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660368/
关键词：
Sheep Cold Exposure Glucose Metabolism Glucose Clamp Technique Feeding Insulin Glucagon Blood Flow グルコースめん羊後肢

项目摘要

Isotope dilution using [U-^<13>C] glucose was combined with measurement of hindquarters arteriovenous difference and blood flow to examine the effect of cold exposure on whole-body and net hindquarters glucose metabolism in sheep. Determinations were carried out in a thermoneutral environment and on day 5 of mild cold exposure. Whole-body blood glucose turnover rate was 1.56 and 1.91 mg kg^<-1> min^<-1> in the thermoneutral environment and during cold exposure, respectively. Net hindquarters glucose uptake was 0.79 and 1.19 mg kg^<-1> min^<-1>, corresponding to 50 and 61% of whole-body turnover rate, respectively. These results suggest that in sheep the contribution of glucose uptake by the hindquarters tended to increase during cold exposure, even though whole-body blood glucose turnover rate did not increase significantly.Effects of cold exposure and level of feed intake on blood glucose kinetics, plasma insulin and glucagon responses to feeding and tissue responsiveness and sensitiv … More ity to insulin were measured in sheep. The isotope dilution technique was used for determination of blood glucose turnover rates, and the hyperinsulinemic euglycemic clamp technique was applied for determination of insulin action and sensitivity. Plasma insulin and glucagon responses to feeding were mainly influenced by dietary level and environmental temperature, respectively. Blood glucose turnover rates were greater during cold exposure than in the thermoneutral environment, and tended to be greater for the sheep fed the diet ad libitum. The rates changed little accompanied by feeding. The hyperinsulinemic euglycemic clamp technique was carried out in the thermoneutral environment and on day 8 of cold exposure. The glucose infusion rate (GIR) was greater during cold exposure than in the thermoneutral environment, and were greater for restricted feeding than for ad libitum feeding. The maxmal GIR was also greater during cold exposure, whereas was not influenced by dietary level. Plasma insulin concentrations at the half maxmal GIR tended to be lower during cold exposure than in the thermoneutral environment, and did not differ between diets. Therefore, insulin action sensitivity were influenced by cold exposure, whereas it could not be obtained the evidence that they were influenced by level of feed intake in sheep fed roughage diet. Less

使用[U-13C]葡萄糖的同位素稀释与后躯动静脉差异和血流量的测量相结合，以检查寒冷暴露对绵羊全身和净后躯葡萄糖代谢的影响。测定是在热中性环境和轻度寒冷暴露的第 5 天进行的。在热中性环境和冷暴露期间，全身血糖周转率分别为1.56和1.91mg kg^ -1 min^ -1 。后躯葡萄糖净摄取量为0.79和1.19mg kg^-1min^-1，分别对应于全身周转率的50％和61％。这些结果表明，尽管全身血糖周转率没有显着增加，但在寒冷暴露期间，绵羊后躯吸收的葡萄糖的贡献往往会增加。在绵羊中测量了寒冷暴露和采食水平对血糖动力学、血浆胰岛素和胰高血糖素对进食的反应以及组织对胰岛素的反应性和敏感性的影响。同位素稀释技术用于测定血糖周转率，高胰岛素正常血糖钳夹技术用于测定胰岛素作用和敏感性。血浆胰岛素和胰高血糖素对喂养的反应主要分别受饮食水平和环境温度的影响。暴露在寒冷环境中的血糖周转率高于中性环境中的血糖周转率，并且对于随意喂养的羊来说，血糖周转率往往更高。随着喂养的进行，速率变化不大。高胰岛素正常血糖钳夹技术在热中性环境和冷暴露第 8 天进行。寒冷暴露期间的葡萄糖输注率（GIR）高于热中性环境中的葡萄糖输注率（GIR），并且限制喂养的葡萄糖输注率高于随意喂养的葡萄糖输注率（GIR）。冷暴露期间最大 GIR 也更大，但不受饮食水平的影响。半最大 GIR 时的血浆胰岛素浓度在寒冷暴露期间往往低于热中性环境中，并且在饮食之间没有差异。因此，胰岛素作用敏感性受到寒冷暴露的影响，但无法获得证据表明其受到饲喂粗饲料的羊采食量水平的影响。较少的