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Nutritional Regulation of Protein Synthesis in Ruminants

反刍动物蛋白质合成的营养调节

基本信息

批准号：
11660277
负责人：
SANO Hiroaki
金额：
$ 2.3万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660277/
关键词：
Ruminant Goat Protein Synthesis Energy Amino Acid Glucose Turnover Rate Stable Isotope Compound 代謝回転速度デンプン

项目摘要

Homeotasis of animals is maintained by coordination of protein, carbohydrate and lipid metabolism. For the efficient production of ruminants, therefore, it is important to clarify the relationship between protein synthesis and other nutrient metabolism. In this experiment, the effects of intake and source of energy on metabolism of blood glucose and amino acid, protein synthesis and their relationship were measured. Mature goats were used in this study. They were fed aflalfa hay cubes, ground corn and soybean meal as a basal diet. In this study, three experiments were conducted. They were (1) starch supplementation, (2) non-protein intake and (3) source of carbohydrate. For each experiment, the isotope dilution methods of [U-^<13>C]glucose, [^2H_5]phenylalanine and [^2H_2]tyrosine were conducted and the turnover rates were determined. Protein synthsis rate was estimated from turnover rate of phenylalanine and its oxidation rate to tyrosine. Turnover rates of blood phenylalanine, tyrosine and glucose and protein synthesis increased with increasing energy intake. They were influenced little by source of carbohydrate. Therefore, it was suggested that in goats protein synthesis and nutrient metabolism are influenced by energy intake but not by source of energy.

动物的稳态平衡是通过蛋白质、碳水化合物和脂质代谢的协调来维持的。因此，研究反刍动物蛋白质合成与其他营养物质代谢的关系，对于反刍动物的高效生产具有重要意义。本实验测定了不同能量摄入和能量来源对血糖、氨基酸代谢和蛋白质合成的影响及其相互关系。本研究使用成年山羊。他们被喂食aflalfa干草块，玉米粉和大豆粉作为基础饮食。在这项研究中，进行了三个实验。它们是（1）补充淀粉，（2）非蛋白质摄入量和（3）碳水化合物来源。在每个实验中，进行[U-^<13>C]葡萄糖、[^2H_5]苯丙氨酸和[^2H_2]酪氨酸的同位素稀释法，并测定转换率。蛋白质降解速率由苯丙氨酸的转化率和氧化为酪氨酸的速率估算。血液苯丙氨酸、酪氨酸、葡萄糖和蛋白质合成的周转率随能量摄入的增加而增加。碳水化合物来源对它们的影响不大。因此，山羊蛋白质合成和营养物质代谢受能量摄入的影响，而不受能量来源的影响。