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Effects of environmental temperature on functional morphology of avian pineal gland and gonad.

环境温度对禽松果体和性腺功能形态的影响。

基本信息

批准号：
07660401
负责人：
OHSHIMA Koji
金额：
$ 1.34万
依托单位：
SHINSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

The present study focuses on ultrastructure, acid phosphatase (AcPase) activity of lysosomes and serotonin immunoreactivity in the pineal organs of normal and cold-exposed chickens. Although the pinealocytes of control and cold-exposed chickens possessed lysosome-like dense bodies, they had scarcely any dense-cored vesicles which were typically present in the secretory rudimentary photoreceptor cells of the lacertilian pineal organ. The pinealocytes of the cold-exposed chicken had an invaginated nucleus containing small indistinct nucleoli and smaller number of mitochondria and lysosome-like dense bodies than those of the control chickens. At the ultrastructural level, the AcPase activity was demonstrated in most of these dense bodies in the pinealocytes, which were regarded as lysosomes. Whereas the serotonin immunoreactivity was shown to be localized on a small number of these dense bodies, especially on large ones, and occasionally on the circumference of lipid-like inclusions in the pinealocytes. These results suggested that a part of the dense bodies in the pinealocytes are involved in the indoleamine metabolism of the chicken pineal organ, and they may have functional relationships with the lysosomes. Furthermore, the serotonin immunoreactivity and AcPase activity of the pinelocytes in the cold-exposed chickens were slightly reduced by comparison with those in the control chickens. However, these cytochemical changes owing to cold-exposure were less distinct than those found in seasonal variations of the Japanese lizard pineal (Hirai and Ohshima, 1993). Therefore, the environmental temperature may not have a more drastic effect on the secretory function in the chicken pinealocytes.

本研究对正常鸡和冷暴露鸡松果体的超微结构、溶酶体酸性磷酸酶活性和5-羟色胺免疫反应进行了研究。虽然对照组和冷暴露鸡的松果体细胞具有溶酶体样致密体，但它们几乎没有任何致密核心囊泡，这些囊泡通常存在于lacertilian松果体器官的分泌性原始感光细胞中。冷暴露鸡的松果体细胞核内陷，核仁小而不明显，线粒体和溶酶体样致密体数量少于对照鸡。在超微结构水平上，松果体细胞内的致密体大多数都具有腺苷酸活性，这些致密体被认为是溶酶体。而5-羟色胺的免疫反应被证明是本地化的这些致密体的少数，特别是在大的，偶尔在周围的脂质样包涵体的松果体细胞。这些结果表明，鸡松果体细胞内的致密体部分参与了鸡松果体的吲哚胺代谢，并与溶酶体有一定的功能联系。此外，冷暴露鸡的5-羟色胺免疫反应性和腺苷酸反应性的松质细胞在冷暴露的鸡与对照组相比，略有下降。然而，这些细胞化学变化，由于冷暴露是不明显的比那些发现在季节变化的日本蜥蜴松果体（平井和大岛，1993年）。因此，环境温度对鸡松果体细胞分泌功能的影响可能并不显著。