Regulation of blood pressure by glomus cells distributed in the artery

分布在动脉中的血管球细胞对血压的调节

• 批准号: 07670031
• 负责人: KAMEDA Yoko
• 金额: $ 1.41万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670031/
• 关键词: Chicken; Carotid body; Common carotid artery; Glomus cells; Neuropeptide Y; Accessory carotied body; Immunohistochemistry; Northern blot; ノーザンブロット; C型心房性ナトリウム利尿ペプチド

In the chicken, the thyroid gland, cranial and caudal parathyroid glands, carotid body and ultimobranchial gland are situated as a continuous series along the common carotid artery. The organs are supplied with branches that arise by one trunk from the middle portion of the common carotid artery. The glomus cells of the chicken carotid body express intense immunoreactivity for serotonin. Furthermore, serotonin-immunoreactive cells are widely ditributed in the wall of the common carotid artery and its branches supplying the endocrine organs (Kameda, 1990a). I have previously reported at the light microscopic level that glomus cells located in the wall of the common carotid artery and its branches express immunoreactivity for neuropeptide Y (NPY) (Kameda, 1990b). The present study examined the localization of NPY in the glomus cells at the electron microscopic level with the postembedding immunogold labeling. Gold particles representing NPY immunoreactivity were located exclusively on the secretory granules of the glomus cells. Furthermore, NPY gene expression was studied by means of in situ hybridization and northern blot analysis. In situ hybridization study with digoxigenin-labeled synthetic oligonucletotide probes from chicken NPY exon 2 indicated that the distribution of labeled cells was similar to that with immunohistochemistry. In addition, the data were supported by northern blot analysis with a chicken NPY exon 4 probe. mRNA from chicken common carotid artery and its branches was extracted and poly (A) ^+RNA was purified. The specific band for NPY gene was obtained in the extracts. These results provide the evidence that glomus cells distributed in the common carotid artery and its branches systhesize and secrete NPY.It is well known that NPY is a potent vasoconstrictor. The glomus cells in and around arteries may be involved in the regulation of arterial blood flow.

鸡的甲状腺、头侧和尾侧甲状旁腺、颈动脉体和后鳃腺沿着颈总动脉排列成一个连续的序列。这些器官由来自颈总动脉中部的一个主干的分支供应。鸡颈动脉体的球细胞表达强烈的5-羟色胺免疫反应性。此外，在供应内分泌器官的颈总动脉及其分支的壁中广泛分布有降钙素免疫反应细胞（Kameda，1990 a）。我以前曾在光镜水平上报道过，位于颈总动脉及其分支壁中的血管球细胞表达神经肽Y（NPY）的免疫反应性（Kameda，1990 b）。本研究应用免疫金标记技术，在电镜水平上观察了神经肽Y在血管球细胞中的定位。代表NPY免疫反应性的金颗粒仅位于球细胞的分泌颗粒上。用原位杂交和北方印迹分析方法研究NPY基因的表达。用地高辛标记的鸡NPY外显子2合成寡核苷酸探针进行原位杂交研究表明，标记细胞的分布与免疫组织化学相似。此外，用鸡NPY外显子4探针进行的北方印迹分析也支持该数据。提取鸡颈总动脉及其分支的mRNA，纯化poly（A）^+RNA。提取物中获得了NPY基因的特异性条带。这些结果为分布于颈总动脉及其分支的血管球细胞合成和分泌NPY提供了证据。动脉及其周围的球细胞可能参与动脉血流的调节。

项目成果

Kameda, Y.: "Co-expression of vimentin and 19S-thyroglobulin in follicular cells located in the C-cell complex of dog thyroid gland" J.Hitochem.Cytochem.43. 1097-1106 (1995)

Kameda, Y.：“位于狗甲状腺 C 细胞复合体中的滤泡细胞中波形蛋白和 19S-甲状腺球蛋白的共表达”J.Hitochem.Cytochem.43。

Kameda, Y.: "Evidence to support the distal vagal ganglion as the origin of C cells of the ultimobranchial gland in the chick" J.Comp.Neurol.359. 1-14 (1995)

Kameda, Y.：“支持远端迷走神经节作为雏鸡上鳃腺 C 细胞起源的证据”J.Comp.Neurol.359。

Kameda, Y.: "Differential distribution of S-100 protein and vimentin in the hypophseal pars tuberalis of the guinea pig" J.Histochem.Cytochem.44. 501-510 (1996)

Kameda, Y.：“S-100 蛋白和波形蛋白在豚鼠垂体结节部中的差异分布”J.Histochem.Cytochem.44。

Kameda,Y.: "Differential distribution of S-100 protein and vimentin in the hypophyseal pars tuberalis of the guinea pig" J.Histochem.Cytochem.44. 501-510 (1996)

Kameda,Y.：“豚鼠垂体结节部 S-100 蛋白和波形蛋白的差异分布”J.Histochem.Cytochem.44。

Kameda, Y.: "Ultrastructural immunogold localization of vimentin and S-100 protein in guinea pig pars tuberalis" J.Histochem.Cytochem.44. 511-518 (1996)

Kameda, Y.：“豚鼠结节部波形蛋白和 S-100 蛋白的超微结构免疫金定位”J.Histochem.Cytochem.44。