EFFECTS OF SEX HORMONES ON BURSA OF FABRICIUS,WITH SPECIAL REFERENCE TO BURSIN-mRNA and B CELL APOPTOSIS.

性激素对法氏囊的影响，特别是法氏囊蛋白mRNA和B细胞凋亡。

• 批准号: 07670037
• 负责人: SAKABE Kou
• 金额: $ 0.9万
• 依托单位: TOKAI UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670037/
• 关键词: bursa of Fabricius; sex steroid; B lymphocytes; apoptosis; bursin; ブルソポペチン; Bリンパ球分化

Structural changes in the bursa of Fabricius of white Leghorns after the intraperitoneal injection with high dose (100mg/kg) of androgen, estrogen and cortisone were examined. A large number of reticular epithelial cell (REC) -like structures that contained apoptotic B cells were observed. The majority of apoptotic B cell-containing REC-like structures had an epithelial nature and were mainly located in the corticomedullary region and, in part, in medulla. Most B cells seemed to undergo apoptosis synchronously in every REC-like structure, but one or two fully intact mature B cells were found in every one. By 5 days after steroid treatment, many vacuoles that contained apoptotic cells in various stages of degeneration were observed within the epithelium. We herein demonstrated that epithelium of REC-like structure had a weak phagocytic activity in the digestion of apoptotic B cells by the presence of phagolysosomes. Futhermore, we found that steroid treatment caused a progressive loss of the number of bursin-mRNA positive REC-like cells. The above results clearly indicate that steroids can induce B cell apoptosis through these hormone directly modulate RECs to produce the bursopoietic hormone, such as bursin.

本文观察了白色来航鸡腹腔注射高剂量（100 mg/kg）雄激素、雌激素和可的松后腔上囊结构的变化。观察到大量网状上皮细胞（REC）样结构，其中含有凋亡的B细胞。大多数凋亡B细胞的REC样结构具有上皮性质，主要位于皮髓质区，部分位于髓质。多数B细胞似乎在每个REC样结构中同步发生凋亡，但在每个REC样结构中发现一个或两个完全完整的成熟B细胞。类固醇治疗后5天，在上皮内观察到许多空泡，其中含有处于不同变性阶段的凋亡细胞。我们在此证明，REC样结构的上皮细胞在吞噬溶酶体的存在下消化凋亡B细胞时具有弱的吞噬活性。此外，我们发现类固醇治疗引起囊蛋白mRNA阳性REC样细胞数量的进行性损失。上述结果表明，类固醇激素可通过直接调节RECs产生囊素等囊生成激素而诱导B细胞凋亡。

项目成果

N. Sakai, K. Sakabe, W. He, K. Seiki: "Sex steroid hormones affect cytoplasmic signal-generation in PHA-stimulated T lymphocytes." Medical Science Research. 23. 839-841 (1995)

N. Sakai、K. Sakabe、W. He、K. Seiki：“性类固醇激素影响 PHA 刺激的 T 淋巴细胞的细胞质信号生成。”

Issei Kawashima,Kou Sakabe,Akira Akatsuka and Kanji Seiki: "Effects of estrogen on female mouse thymus,with apecial reference to ER-mRNA and T cell subpopulations." Pathophysiol.2. 235-241 (1995)

Issei Kawashima、Kou Sakabe、Akira Akatsuka 和 Kanji Seiki：“雌激素对雌性小鼠胸腺的影响，特别涉及 ER-mRNA 和 T 细胞亚群。”

R. Urano, K. Sakabe , K. Seiki, M. Ohkido: "Female sex hormone stimulates cultured human keratinocyte proliferation and its RNA-and protein-synthetic activities" Journal of Dermatological Science. 9. 176-184 (1995)

R. Urano、K. Sakabe、K. Seiki、M. Ohkido：“女性性激素刺激培养的人类角质形成细胞增殖及其 RNA 和蛋白质合成活性”《皮肤病学杂志》。

Kou Sakabe,Kanji Seiki,Norie Sakai and Wei He: "Establishment of ‘crosstalk'between the thymus and brain at an carly stage of Tetal life in the rat." Med.Sci.Res.24. 439-442 (1996)

Kou Sakabe、Kanji Seiki、Norie Sakai 和 Wei He：“大鼠 Tetal 生命早期阶段胸腺和大脑之间‘串扰’的建立。”Med.Sci.Res.24 (1996)。

Issei Kawashima,Kou Sakabe,Akira Akatsuka and Kanji Seiki: "Effects of estrogen on female mouse thymus,with special reference to ER-mRNA and T cell subpopulations." Pathophysiol.2. 235-241 (1995)

Issei Kawashima、Kou Sakabe、Akira Akatsuka 和 Kanji Seiki：“雌激素对雌性小鼠胸腺的影响，特别是 ER-mRNA 和 T 细胞亚群。”