EFFECTS OF SEX HORMONE AND ENVIRONMENTAL ENDOCRINE DISRUPTORS ON GUT-ASSOCIATED LYMPHOID TISSUES

性激素和环境内分泌干扰物对肠道相关淋巴组织的影响

• 批准号: 11670023
• 负责人: SAKABE Kou
• 金额: $ 1.41万
• 依托单位: The Kitasato Institute (2000-2001)Tokai University (1999)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670023/
• 关键词: GUT-ASSOCIATED LYMPHOID SYSTEM; ESTROGEN; ENVIRONMENTAL HORMONES; PEYER'S PATCH; LYMPHOCYTES; APOPTOSIS; 7A6 ANTIGEN; IMMUNE DISRUPTQR; 性ホルモン; 環境シグナル; ホルモン様化学物質; 免疫毒性

The effect of environmental endocrine disruptors (EDC)on the immune organ (Peyer s patch) from castrated female mice was examined by molecular biologic, microscopic and flow cytometric techniques.Our findings were as follows : (a) using a monoclonal antibody to estrogen receptor-alpha (ER), ER level in the EDC-treated Peyer's patch was almost 1.2〜2.5 times that in oil-treated controls, respectively ; (b) an electron microscopic observation indicated EDC treatment to bring about apoptosis of lymphocytes which were embraced by stromal cells (i.e. macrophages) ; (c) flow cytometric analysis demonstrated EDC to induce the change of lymphocyte subsets : an increase in the suppressor/cytotoxic (CD4-CD8+) cells with decrease in the helper/inducer (CD4+CD8-) cells in Peyer s patch.It follows from the above findings that EDC may cause morphologic changes in the murine immune organs closely related to T cell function. In addition, these changes appear to derive mainly from EDC-induced tissuespeciic gene expression. The results of the present study suggest that we must recognize the possibility that a lowdose EDC can affect the various immune responses in the capacity of immune disruptors.

环境内分泌干扰物（EDC）对免疫器官的影响应用分子生物学、显微镜和流式细胞术等技术对去势雌性小鼠Peyer’s斑进行了研究，结果表明：（1）雌激素受体α（ER）单克隆抗体检测表明，经EDC处理的Peyer’s斑的ER水平分别为对照组的1.2 ~ 2.5倍;（B）电子显微镜观察表明EDC处理导致被基质细胞包围的淋巴细胞凋亡（c）流式细胞术分析表明EDC诱导淋巴细胞亚群的变化：抑制/细胞毒性增加结果表明，EDC可引起与T细胞功能密切相关的免疫器官形态学改变。此外，这些变化似乎主要来自EDC诱导的组织特异性基因表达。本研究的结果提示，我们必须认识到低剂量EDC可能以免疫干扰物的身份影响各种免疫反应。

项目成果

Y.Nito,K.Sakabe,H.Yamauchi,H.Aikawa: "Fetal brain damage induced by acute arsenic poisoning in pregnanncy in rats."Biomed.Res.Trace Elements. 11(3). 300-305 (2000)

Y.Nito、K.Sakabe、H.Yamauchi、H.Aikawa：“大鼠妊娠期急性砷中毒引起胎儿脑损伤。”Biomed.Res.Trace Elements。

Kou Sakabe: "Effect of environmental signals on bursa of Fabricius, with special reference to B celL apoptosis"International Journal of Immunopharmacology. 22(in press). (2000)

Kou Sakabe：“环境信号对法氏囊的影响，特别是 B 细胞凋亡”国际免疫药理学杂志。

井上泰泉: "環境ホルモンハンドブック"講談社. 315 (1999)

井上大仙：《环境激素手册》讲谈社315（1999）。

K.Sakabe,T.Yoshida,H.Aikawa,S.Izumi,Y.Osamura,F.Kayama: "Inhibitory effect of environmental endocrine disruptors on thymosin 1 production in thymus epithelial culture."Altern.Animal Test.Experiment.. 6(4). 157-159 (2000)

K.Sakabe,T.Yoshida,H.Aikawa,S.Izumi,Y.Osamura,F.Kayama：“环境内分泌干扰物对胸腺上皮培养物中胸腺素 1 产生的抑制作用。”Altern.动物测试.实验.. 6

K.Sakabe,M.Miyata,S.Ishikawa,Y.Aizawa: "Effect of environmental endocrine disruptors on the proliferation of Human breast cancer cells in a culture model : a role of non-genomic action"Endocr.Disrupt.Res.. 3. 165-166 (2000)

K.Sakabe，M.Miyata，S.Ishikawa，Y.Aizawa：“环境内分泌干扰物对培养模型中人乳腺癌细胞增殖的影响：非基因组作用的作用”Endocr.Disrupt.Res..