WHY LYMPHOCYTES CAN MIGRATE FREELY IN THE LYMPHOID ORGANS?

为什么淋巴细胞可以在淋巴器官中自由迁移？

• 批准号: 07670045
• 负责人: TAKAHASHI Kenichi
• 金额: $ 0.38万
• 依托单位: OSAKA PREFECTURAL COLLEGE OF HEALTH SCIENCES
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670045/
• 关键词: THYMUS; LYMPHOCYTE; ADHESION; CADHERIN; TENASCIN

It is well known that adhesive substances such as cadherin are participated in the cell-cell contact. However, it is not clear whethrer the lymphocytes which function in the lymphoid organs, or bone marrow, thymus, lymph node, and spleen, and circulate freely in the blood express such adhesive substances.In the present study, the rat thymus was fragmented in Hanks solution and centrifuged. The supernatant was cultured with adhesive hepatoma cell line. As a result, hepatoma cells contact with each other, but didn't with dish surface, or substrate, suggesting that spreading-inhibiting factors may be contained in the supernatant.I would like to promote the study on tenascin which is a large extracellular matrix protein expressed in tissues of the immune system, including the bone marrow, thymus and the T cell areas of secondary lymphoid organs, and plays an important role as an adhesive inhibitor.

众所周知，粘附物质如钙粘蛋白参与细胞-细胞接触。然而，在淋巴器官或骨髓、胸腺、淋巴结和脾脏中起作用并在血液中自由循环的淋巴细胞是否表达这种粘附物质尚不清楚。将上清液与肝癌细胞株贴壁培养。因此，肝癌细胞相互接触，但不与培养皿表面或基质接触，这表明上清液中可能含有扩散抑制因子。我希望促进对Tenascin的研究，Tenascin是一种在免疫系统组织中表达的大型细胞外基质蛋白，包括骨髓，胸腺和次级淋巴器官的T细胞区，并且作为粘附抑制剂起重要作用。

项目成果

TSUGAWA,K.: "Association of hnRNP S1 proteins C2 and D2 with vimentin intermediate filaments" CELL STRUCTURE & FUNCTION. 22. 239-246 (1997)

TSUGAWA,K.：“hnRNP S1 蛋白 C2 和 D2 与波形蛋白中间丝的关联”细胞结构

INOUE,A.: "Molecular Cloning of a RNA binding protein, S1-1" NUCLEIC ACIDS RESEARCH. 24. 2990-2997 (1996)

INOUE,A.：“RNA 结合蛋白 S1-1 的分子克隆”核酸研究。

Inoue, A., K.P.Takahashi, M.Kimura, T.Watanabe and S.Morisawa: "Molecular cloning of a RNA binding protein, S1-1" Nucleic Acids Reseach. 24・15. 2990-2997 (1996)

Inoue, A.、K.P.Takahashi、M.Kimura、T.Watanabe 和 S.Morisawa：“RNA 结合蛋白的分子克隆，S1-1”《核酸研究》24·15（1996）。

A.Inoue, K.P.Takahashi et al.: "Molecular cloning of a RNA binding protein, S1-1" Nucleic Acids Rsearch. 24(15). 2990-2997 (1996)

A.Inoue、K.P.Takahashi 等人：“RNA 结合蛋白的分子克隆，S1-1”核酸研究。

Tsugawa, K., K.P.Takahashi, T.Watanabe, A.Mai, D.Fujimoto and A.Inoue: "association of hnRNP S1 proteins C2 and D2 with vimentin intermediate filaments" Cell Structure and Function. 22. 239-246 (1997)

Tsukawa, K.、K.P.Takahashi、T.Watanabe、A.Mai、D.Fujimoto 和 A.Inoue：“hnRNP S1 蛋白 C2 和 D2 与波形蛋白中间丝的关联”细胞结构和功能。