Relationship among blood pressure, tail blood flow and plasma catecholamine levels in chronic spinal rats.

慢性脊髓大鼠血压、尾部血流量与血浆儿茶酚胺水平的关系。

• 批准号: 07670087
• 负责人: TSUCHIYA Katsuhiko
• 金额: $ 1.47万
• 依托单位: Institute of Tropical Medicine Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670087/
• 关键词: chronic spinal rat; blood pressure and heart rate; sympathetic nerves; plasma catecholamine; HPLC; tail skin blood vessels; isoproterenol; adrenal medulla; ノルアドレナリン; アドレナリン; 尾部血管; 血圧心拍; プロプラノロール; 心拍; 血圧

In chronic spinal rats, relationship among the systemic blood pressure (BP), the tail blood flow and plasma catecholamines, noradrenaline (NA) and adrenaline (AD). Spinal cord of adult Wistar Kyoto rat (WKY) was transected at lower cervical portion, and the rats were reared as chronic spinal rats in a warm room (30ﾟC). The systemic blood pressure (BP) was measured by the tail cuff method, in other cases BP was measure by a pressure transducer at the femoral artery. Heart rate (HR) was calculated by of R-R interval of a ECG.Blood sample was drawn through the cannula of the jugular vein under conscious condition. Plasma level of NA and AD were estimated by HPLC.RESULTS : A : Effect of the acute spinalization : Spinal cord was transected at level of Th1 (Group-A) or C7 (Group-B) of the vertebral segment. In Group-A rats, Th1 spinal nerve remain intact which contain the cardiac sympathetic nerve fibers (Waibl H,1973). In Group-A,acute spinalization induced a sustained increase of HR and co … More re temperature gradually increased after the spinalization, BP decreased slightly. Only Group-A rats survived after the spinaliztion. B : Pharmacological studies of blood vessels of the tail. Intermediate condition (not constricted but not fully dilated) was kept in chronic spinal rats. Either propranolol (beta-adrenergic antagonist, 8 mg/kg) or isoproterenol (beta-adrenergic agonist, 0.1 mg/kg) induced a transient tail vasoconstriction. C : plasma level of NA and AD : By the spinal transection, systemic BP was decreased from 120 mmHg to 80 mmHg. BP recovered gradually day by day but did not attained level of normal value as reported by Osborn (Am J Physiol, 1989). Mean NA level (mean <plus-minus> S.E) was 50<plus-minus>32 pg/ml (N=6) in the 1st week (W) after the spinalization, and 147<plus-minus>42 pg/ml (N=7) in the 2nd W.Value in the 2nd W was significantly (P<0.05) high compared to value of 1st W.Mean AD was 594<plus-minus>324 pg/ml in the 1st W and 309<plus-minus>153 pg/ml in the 2nd W,there was no significant difference between these values. From these results, it is suggested that humoral factors from adrenal medulla and others are more important for the regulation of the blood vessels in chronic sp Less

慢性脊髓大鼠体压、尾血流量与血浆儿茶酚胺、去甲肾上腺素和肾上腺素的关系取成年Wistar Kyoto大鼠（WKY）下颈段脊髓横断，作为慢性脊髓大鼠饲养于温室（30℃）。全身血压（BP）采用尾袖法测量，其他情况下采用股动脉压力传感器测量。心率（HR）由心电图R-R间期计算。在清醒状态下通过颈静脉插管抽血。采用高效液相色谱法测定血浆NA和AD水平。结果：A：急性脊柱化的影响：脊髓在Th1水平（A组）或C7水平（b组）椎段横切。a组大鼠Th1脊神经保持完整，其中包含心脏交感神经纤维（Waibl H,1973）。在a组，急性刺化诱导HR和co持续升高，刺化后体温逐渐升高，血压略有下降。只有a组大鼠脊髓化后存活。B：尾部血管的药理研究。慢性脊髓大鼠保持中间状态（未收缩但未完全扩张）。普萘洛尔（β -肾上腺素能拮抗剂，8mg /kg）或异丙肾上腺素（β -肾上腺素能激动剂，0.1 mg/kg）均可诱导短暂性尾部血管收缩。C：血浆NA和AD水平：脊髓横断后，全身血压从120 mmHg降至80 mmHg。血压逐渐恢复，但没有达到Osborn （Am J Physiol, 1989）报道的正常值水平。平均NA水平（平均<正负> S.E）为50<正负>32 pg/ml (N=6)，第2周为147<正负>42 pg/ml (N=7)，与第1周相比，第2周的平均AD为594<正负>324 pg/ml，第2周为309<正负>153 pg/ml，差异无统计学意义（P<0.05）。提示肾上腺髓质等体液因子对慢性sp Less血管的调节作用更为重要

项目成果

Tsuchiya K: "Vasoconstriction induced by β-adrenergic antagonist in the tail of chronic spinal rats." Trop Med. 37(4),. 175-183 (1995)

Tsuchiya K：“慢性脊髓大鼠尾部的 β-肾上腺素能拮抗剂诱导的血管收缩。”Trop Med 37(4), 175-183 (1995)。

土屋勝彦: "日々繰り返し拘束刺激のラット体温及び心拍数に及ぼす影響," 日生気誌. 32(3). S50- (1995)

Katsuhiko Tsuchiya：“每日重复约束刺激对大鼠体温和心率的影响”，Nissei Kishi 32(3)。

松本孝朗: "放射鼓膜温計(クイックサーモ)による健常人鼓膜温の検討" 日生気誌. 33(4)(in press). 125-132 (1996)

Takao Matsumoto：“使用辐射鼓膜温度计（Quick Thermo）研究健康受试者的鼓膜温度”Nissei Keizhi 33(4)（出版中）。

土屋勝彦: "日々繰り返し拘束刺激のラット体温及び心拍数に及ぼす影響II" 日生気誌.33(3). S35- (1996)

Katsuhiko Tsuchiya：“每日重复约束刺激对大鼠体温和心率的影响 II”Nissei Kishi.33(3) S35- (1996)。

Matsumoto T: Sweat responses in hyper-thyroidism and hypothyroidism. p157-p160, "Body temperature and metabolism". Ed by Nagasaka T & Milton AS,IPEC Inc, (1995)

Matsumoto T：甲状腺功能亢进症和甲状腺功能减退症的出汗反应。