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Possible involvement of vascular cells-related transcription factors in human inflammatory diseases and cancer tissue.

血管细胞相关转录因子可能参与人类炎症性疾病和癌症组织。

基本信息

批准号：
07670190
负责人：
OHTANI Haruo
金额：
$ 1.47万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670190/
关键词：
Inflammatory Bowel Disease Colon cancer GATA gene in situ hybridization GATA-2 GATA-3 癌炎症 in situ hybridization

项目摘要

Inflammatory diseases and cancer are representative diseases associated with angiogenesis. We report here possible involvement of transcription factors GATA family in these lesions by in situ hybridization. Specimens were sampled from human inflammatory bowel disease and colon cancer. Fresh tissues selected from representative areas were fixed in 4% paraformaldehyde + 0.5% glutaradehyde for 16 h, dehydrated and embedded in paraffin. Digoxigenin-labeled tRNAs were made by in vitro transcription. For GATA-1, no significant results were obtained because of no differences between results with sense and antisense probes. Positive signals were obtained for GATA-2 and -3, Angiogenesis was most remarkable in ulcer bases and in cancer stroma, where GATA-2 and -3 were negative in endothelial cells. GATA-2 and -3 were positive in endothelial cells of venules situated in the peripheral zone of ulcer bases, where mild inflammatory cells were distributed. In cancer tissue, GATA-2 and -3 were positive in some of venules located along the invasive margin. This area is characterized by moderate degree of immune/inflammatory infiltrate composed of lymphocytes, monocytes/macrophages and neutrophils. T-cells in ulcer bases were positive for GATA-2 and -3, which corresponded to the area of high-degree inflammation. In cancer tissue, some cancer cells and stromal fibroblasts were positive for GATA-2 and -3. These results suggested that GATA family is involved in inflammatory changes in inflammatory diseases and that occurring in cancer tissue as one of host reactions. This study further supports our concept that there are similarities between the host reactions in cancer tissue and inflammatory changes.

炎症性疾病和癌症是血管生成的代表性疾病。我们在这里报告可能参与转录因子GATA家族在这些病变的原位杂交。标本取自人类炎症性肠病和结肠癌。选取代表性地区的新鲜组织，在4%多聚甲醛+ 0.5%戊二醛溶液中固定16 h，脱水后包埋石蜡。通过体外转录制备地高辛标记的trna。对于GATA-1，由于正义探针和反义探针的结果没有差异，所以没有得到显著的结果。GATA-2和-3表达阳性，血管生成在溃疡基底和癌间质中最为显著，内皮细胞中GATA-2和-3表达阴性。GATA-2和-3在溃疡基底外周区小静脉内皮细胞中呈阳性，该区域有轻度炎症细胞分布。在癌组织中，GATA-2和-3在沿浸润边缘的一些小静脉中呈阳性。该区域的特征是由淋巴细胞、单核细胞/巨噬细胞和中性粒细胞组成的中等程度的免疫/炎症浸润。溃疡基底t细胞GATA-2和-3阳性，与高度炎症区相对应。在癌组织中，部分癌细胞和间质成纤维细胞中GATA-2和-3呈阳性。这些结果提示GATA家族作为宿主反应之一参与炎症性疾病的炎症变化和肿瘤组织的炎症变化。本研究进一步支持了我们的观点，即肿瘤组织中的宿主反应与炎症变化之间存在相似性。