Clinicopathological analyses on the blood-borne metastasis in colorectal carcinoma

结直肠癌血源性转移的临床病理分析

• 批准号: 11670164
• 负责人: OHTANI Haruo
• 金额: $ 2.05万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670164/
• 关键词: cancer metastasis; Host Reaction; Immune response; Colon Cancer; 大腸がん; CD8陽性リンパ球; 腫瘍免疫; 多変量解析; 血行性転移; マクロファージ

The present study revealed that the number of macrophages distributed along the invasive margin of colorectal cancer is smaller in the groups of synchronous or metachronous hematogenous metastasis than in control group. This demonstrates that macrophages along the invasive margin could be inhibitory to hematogenous metastasis. Furthermore, these macrophages expressed Fas ligand by immunohistochemistry and in situ hybridization. Therefore, we could speculate the induction of apoptosis in cancer cells as one of the mechanisms of the above-mentioned results. Apoptotic cancer cells detected by a monoclonal antibody M30 is present in these macrophages. These macrophages expressed B7-2, MHC class II and ICAM-1, sharing common features with dendritic cells. Therefore, we could speculate that these macrophages present cancer antigens to T cells in the regional lymph nodes. In fact, the number of CD8+ T cells infiltrated within cancer cell nests (intraepithelial type) is a prognostic factor. We would be able to assess the prognosis of cancer -bearing patients when these host factors are considered together with factors of cancer cells.

本研究发现，同步或异时性血行转移组沿结直肠癌浸润缘分布的巨噬细胞数量少于对照组。这表明沿浸润边缘的巨噬细胞可以抑制血液转移。此外，通过免疫组化和原位杂交，这些巨噬细胞表达Fas配体。因此，我们可以推测诱导癌细胞凋亡是上述结果的机制之一。单克隆抗体M30检测到这些巨噬细胞中存在凋亡的癌细胞。这些巨噬细胞表达B7-2、MHC II类和ICAM-1，与树突状细胞具有共同的特征。因此，我们可以推测这些巨噬细胞向局部淋巴结的T细胞呈递癌抗原。事实上，癌细胞巢内浸润的CD8+ T细胞的数量（上皮内型）是一个预后因素。将这些宿主因素与肿瘤细胞因素结合起来考虑，我们就能够评估患癌患者的预后。

项目成果

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Ohtani.H: "Pathophysiologic Significance of host reactions in human cancer rissue"Tohoku J. Exp. Med.. 187. 193-202 (1999)

Ohtani.H：“人类癌症组织中宿主反应的病理生理学意义”Tohoku J. Exp。

Ohtani, H.: "Pathophysiologic significance of host reactions in human cancer tissue Desmoplasia and Tumor Immunity"Tohoku J Exp Med. 187. 193-202 (1999)

Ohtani, H.：“人类癌症组织结缔组织形成和肿瘤免疫中宿主反应的病理生理学意义”Tohoku J Exp Med。

Nakano et al.: "Proliferative activity of intramoral CD8^+ Tcells and a prognostic fqactor"Cancer Res.. 61. 5132-5136 (2001)

Nakano 等人：“道德内 CD8+ T 细胞的增殖活性和预后因子”Cancer Res.. 61. 5132-5136 (2001)

Saito K, Takeha S, Shiiba K. Matsuno S, Sorsa T, Nagura H, Ohtani H: "Clinicopathologic significance of urokinase receptor- and MMP-9-positive stromal cells in human colorectal cancer : Functional multiplicity of matrix degradation on hematogenous metasta

Saito K、Takeha S、Shiiba K. Matsuno S、Sorsa T、Nagura H、Ohtani H：“人结直肠癌中尿激酶受体和 MMP-9 阳性基质细胞的临床病理学意义：血行转移中基质降解的功能多样性