Immunohistochemical Study of Hepatitis Associated Bile Duct Damage in Chronic Hepatitis C,Contrasted with Chronic Bile duct Damage of Primary Biliary Cirrhosis

我们研究了慢性丙型肝炎与原发性胆汁性肝硬化的慢性非化脓性破坏性胆管炎之间的组织学和化学差异。组织学上，前者与淋巴滤泡形成有关，而后者与嗜酸性粒细胞浸润和肉芽肿反应有关。免疫组化结果显示，核粘蛋白（MUC 1、MUC 2、MUC 3和MUC 5/6）和细胞因子（IL-1 α、IL-1 β、IL-2、IL-4、IL-5、IL-8、IFN γ、TNF α、TNF β）的表达谱分别存在差异和相似性。结果表明，两种胆管损伤的发生顺序不同，最近，我们观察到一只常染色体隐性遗传突变小鼠aly/aly，其全身淋巴结缺失，汇管区淋巴样细胞浸润，淋巴滤泡形成，肝内胆管上皮细胞损伤，包括假幽门腺化生和增殖性改变。这些病变中的一些使人联想到原发性胆汁性肝硬化。此外，肝外胆管和肝内大胆管上皮细胞胞浆内含有嗜酸性物质。Aly/Aly小鼠是一种较好的分析胆道物质代谢的动物模型，该模型可能通过免疫调节产生免疫介导的胆管损伤。所以我们继续调查。

We investigated histologically and immunohistochemicaly the differences between hepatitis associated bile duct damage in chronic hapetitis C and chronic non-supprative destructive cholangitis in primary biliary cirrhosis. Histologically, the former was associated with formation of lymphfollicles, in contrast, the latter was associated with eosinophilic infiltration and granulomatous reaction. Immunohistochemically, the expression profile of apomucins (MUC1, MUC2, MUC3 and MUC5/6) and cytokines (IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-8, IFN gamma, TNF alpha, TNF beta) showed differences and similarities respectively. The results suggested the different sequence in the two types of bile duct damage.Recentry, we experinenced an autosomal recessive mutant mouse named aly/aly mouse that lacks systemic lymph nodes and also a variable lymphoid cell infiltration with lymph follicles formation in the portal tract, and variable damages in the intrahepatic biliary epithelial cells including pseudopyloric gland metaplasia and proliferative changes. Some of these lesions were reminiscent of primary biliary cirrhosis. In addition, extrahepatic bile duct and intrahepatic large bile duct contained acidophilic substance in their epithelial cytoplasm. Aly/aly mouse may be a good animal model to analyze the metabolism of the biliary substances, and immune-mediated bile duct damages may be produced in this model by immunological modulations. So we go on the investigation.