DNA ploidy and S-phase fraction of neoplastic and non-neoplastic lesions of the human gallbladder.

人胆囊肿瘤性和非肿瘤性病变的 DNA 倍体和 S 期分数。

• 批准号: 07670214
• 负责人: NAKAMURA Shin-chi
• 金额: $ 1.28万
• 依托单位: School of Medicine Iwate medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670214/
• 关键词: crypt isolation technique; gallbladder; polyp; gallbladder cancer; flow cytometry; DNA content; proliferative activity; S-phase fraction; S-phase fraction; 胆嚢症

Studies on the cell kinetics of the human gallbladder are difficult because of epithelial degeneration by bile. Using the epithelial isolation technique, however, we were able to determine the degree of degeneration and to examine the cell kinetics of gallbladder lesions in freshly resected surgical specimens. Normal and neoplastic epithelia were isolated nonenzymatically from freshly resected gallbladder. The nuclear DNA content and S-phase fraction were estimated in 110 patients with gallbladder lesions by flow cytometry (FCM). Normal tissues and all lesions except carcinomas were diploid. The S-phase fraction of gallstone cases was significantly higher (1.47(]SY+-[)0.70% ; mean(]SY+-[)SD) than normal (0.79(]SY+-[)0.39%) (p<0.0006). All gallbladder carcinomas were multiploid, and their S-phase fraction was 11.63(]SY+-[)3.65%. Cell renewal of normal gallbladder is low. In the gallstone cases, the S-phase fraction was increased, possibly correlated with carcinogenesis.

由于胆汁对胆囊上皮细胞的变性，对胆囊细胞动力学的研究是困难的。然而，使用上皮分离技术，我们能够确定变性的程度，并检查新鲜切除的手术标本中胆囊病变的细胞动力学。从新鲜切除的胆囊中非酶促分离正常和肿瘤上皮。应用流式细胞术（FCM）检测了110例胆囊病变细胞核DNA含量和S期细胞比例。正常组织和除癌外的所有病变均为二倍体。胆囊结石患者的S期细胞比例（1.47 ± 0.70%，平均值± 0.39%）显著高于正常人（0.79 ± 0.39%）（p<0.0006）。胆囊癌均为多倍体，S期细胞比例为11.63 ± 3.65%。正常胆囊细胞更新率低。在胆囊结石病例中，S期分数增加，可能与癌变有关。

项目成果

Y. KITAYAMA: "Expression of p53 and flow cytometric DNA analysis of isolated neoplastic glands of the stomach: an application of the gland isolation method" Virchows Arch. 426. 557-562 (1995)

Y. KITAYAMA：“胃分离肿瘤腺体的 p53 表达和流式细胞术 DNA 分析：腺体分离方法的应用” Virchows Arch。

S Nakamura, J Goto, Y Kitayama, JP Sheffield, IC Talbot: "Flow cytometric analysis of DNA synthetic phase fraction of the normal appearing colonic mucosa in patients with colorectal neoplasms." Gut. 37. 398-401 (1995)

S Nakamura、J Goto、Y Kitayama、JP Sheffield、IC Talbot：“对结直肠肿瘤患者正常结肠粘膜的 DNA 合成相部分进行流式细胞分析。”

S NAKAMURA: "Flow cytometric analysis of DNA symthetic phase fraction of the normal appearing colonicmucosa in patients with colorectal neoplasms" Gut. 37. 398-401 (1995)

S NAKAMURA：“结直肠肿瘤患者正常结肠粘膜 DNA 合成相部分的流式细胞术分析”Gut。

Y Kitayama, H Sugimura, M tanaka, S Nakamura, I Kino: "Expression of p53 and flow cytometric DNA analysis of isolated neoplastic glands of the stomach : an application of the gland isolation method." Virchows Arch. 426. 557-562 (1995)

Y Kitayama、H Sugimura、M tanaka、S Nakamura、I Kino：“胃分离肿瘤腺体的 p53 表达和流式细胞术 DNA 分析：腺体分离方法的应用。”

Y Kitayama: "Cytophotometric and flow cytometric DNA content of isolated glands in gastic neoplasia." Gut. 36. 516-521 (1995)

Y Kitayama：“胃肿瘤中分离腺体的细胞光度法和流式细胞术 DNA 含量。”