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Analysis of the intracellular regulatory mechanism of bcl-2 related apoptosis.

bcl-2相关细胞凋亡的细胞内调控机制分析

基本信息

批准号：
07670238
负责人：
TANAKA Shigeki
金额：
$ 1.28万
依托单位：
MIE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670238/
关键词：
bcl-2 Apoptosis Reactive Oxygen Species Mitochondrial Membrane Potential ミトコンドリア膜透過電位 Fas TNF-α

项目摘要

1. Anti-apoptotic effect and the expression of p26 bcl-2 We have investigated the anti-apoptotic effect of bcl-2 proto-oncogene using bcl-2 transfectants (bcl-2 was introduced to CCRF-CEM,MBC-1, WEHI-164,2AD4) with various factor inducible apoptosis. The cells expressing a large amount of p26 bcl-2 showed higher viability when cell death was induced with H_2O_2, Dexamethasone and anti-lgM.Especially, bcl-2 has a stronger anti-apoptotic effect in the death induced with H_2O_2 than in the death induced with others. There was no significant correlation between the anti-apoptotic effect and the expression of p26 bcl-2 in the cell death induced with A23187, TNF-alpha. 2. Intarcellular events in the early phase of apoptosis and the expression of p26 bcl-2 The studies of intracellular reactive oxygen species and mitochondrial transmembrane potential of MBC-1 cells using fluorescence dye revealed no significant difference between bcl-2 transfectants and controls when cell death was induced with H_2O_2. Whereas p26 bcl-2 has significant anti-apoptotic effect in MBC-1 cells undergoing apoptosis with H_2O_2. When the transfectants of CEM-C7 cells were treated with H_2O_2, reactive oxygen species were markedly reduced but not correlate with the level of p26 bcl-2. Both intracellular reactive oxygen species and mitochondrial transmembrane potential showed no significant change in CEM,MBC-1 cells when cell death was induced with dexamethasone and anti-lgM respectively. These results suggest the anti-apoptotic effect of p26 bcl-2 has some direct relation to reactive oxygen species in early phase of apoptosis.

1. bcl-2转染CCRF-CEM、MBC-1、WEHI-164、2AD4后，通过多种因子诱导细胞凋亡，研究了bcl-2原癌基因的抗凋亡作用。H_2O_2、地塞米松和抗lgm诱导细胞死亡时，大量表达p26bcl -2的细胞存活率较高。bcl-2在H_2O_2诱导的大鼠死亡中具有较强的抗凋亡作用。在A23187、tnf - α诱导的细胞死亡中，抗凋亡作用与p26bcl -2的表达无显著相关性。2. 细胞凋亡早期细胞内事件及p26的表达bcl-2荧光染色研究发现，H_2O_2诱导细胞死亡时，bcl-2转染物与对照组细胞内活性氧和线粒体跨膜电位无显著差异。而p26 bcl-2对H_2O_2诱导的凋亡的MBC-1细胞有明显的抗凋亡作用。H_2O_2处理后，CEM-C7细胞的活性氧含量明显降低，但与p26bcl -2水平无关。地塞米松和抗lgm分别诱导CEM、MBC-1细胞死亡时，细胞内活性氧和线粒体跨膜电位均无明显变化。提示p26bcl -2的抗凋亡作用与细胞凋亡早期活性氧含量有直接关系。