Cell Adhesion Molecules and Growth Factors for Cytodifferentiation and Proliferation of Pulmonary Endocrine Cells

用于肺内分泌细胞细胞分化和增殖的细胞粘附分子和生长因子

• 批准号: 07670254
• 负责人: ITO Takaaki
• 金额: $ 1.47万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670254/
• 关键词: lung; (neuro) endocrine cell; differentiation; morphogenesis; histochemistry; growth factors; cell adhesion molecules; organ culure; 組織細胞化学; アンチセンスオリゴヌクレオチド

In the present study, we investigated the effects of adhesion molecules and growth factors on cytodifferentiation and proliferation of pulmonary endocrine cells (PECs) in cultured fetal lung of hamster and mouse.1. Histochemical studies revealed that PECs are positive for neural cell adhesion molecule (NCAM), GTP-binding protein (Go), calbindinD28k, synaptotagmin and related proteins.2. Fetal hamster lung (at gestational day 11) and fetal mouse lung (atgestational day 13) showed a few airway branches and epithelia were composed of immature epithelial cells. PECs occurred from the immature epithelial cells by 2 days cultivation.3. Anti-NCAM antibody inhibited occurence of PECs and perturb slightly morphogenesis in cultured lung explant of hamster, but anti-E-cadherin antibody did not show any effects.4. Antisense oligonucleotides for NCAM,E-cadherin and epidermal growth factor (EGF) did not affect the morphogenesis and endocrine cell differentiation in cultured hamster lung.5. When growth factors including EGF,acidic FGF,basic FGF,KGF,NGF,TGF-b, HGF,PDGF-AA,PDGF-BB,IGF-I,IGF-II,and amphiregulin were added in the culture medium, morphogenesis of mouse lung explant was inhibited by basic FGF,KGF and TGF-b and the number of PECs decreased by addition of EGF,acidic FGF,basic FGF and KGF.In conclusion, cell adhesion molecules and growth factors can play important roles for development of fetal PECs.

本研究探讨粘附分子和生长因子对培养的仓鼠和小鼠胎肺内分泌细胞（佩奇）分化和增殖的影响.组织化学研究显示，佩奇表达神经细胞粘附分子（NCAM）、GTP结合蛋白（Go）、钙结合蛋白D28 k、突触结合蛋白（synaptotagmin）等相关蛋白.胎鼠肺（胎龄13天）和胎鼠肺（胎龄11天）可见少量气道分支，上皮细胞由未成熟的上皮细胞组成。培养2d，未成熟上皮细胞出现佩奇.抗NCAM抗体可抑制体外培养的仓鼠肺组织中佩奇的发生，并对肺组织的形态发生有一定的干扰作用，而抗E-cadherin抗体则无此作用. NCAM、E-cadherin和EGF的反义寡核苷酸对培养的仓鼠肺的形态发生和内分泌细胞分化无影响.在培养液中加入EGF、酸性FGF、碱性FGF、KGF、NGF、TGF-b、HGF、PDGF-AA、PDGF-BB、IGF-I、IGF-II和双调蛋白等生长因子后，碱性FGF、KGF和TGF-b抑制小鼠肺组织的形态发生，加入EGF、酸性FGF、碱性FGF和KGF后肺上皮佩奇数量减少。

项目成果

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Ito T,Ohyama K,Kusano T,Usuda Y,Nozawa A,Hayashi H,Ohji H,Kitamura H,Kanisawa M: "Pulmonary endocrine cell hyperplasia and papilloma in rats induced by intratracheal injections of extract from particulate air pollutants" Exp Toxicol Pathol. 49 (4). 378-38

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Ito T,Nozawa A,Usuda Y,Kitamura H,Kanisawa M.: "Pulmonary endocrine cells with neural cell adhesion molecule (NCAM) immunostainings" Histochem Cell Biol. 104 (4). 357-362 (1995)

Ito T、Nozawa A、Usuda Y、Kitamura H、Kanisawa M.：“具有神经细胞粘附分子 (NCAM) 免疫染色的肺内分泌细胞” Histochem Cell Biol。