Study of lung epithelial and lung cancer stem cells of the lung-specific gene-targeted mice ; analysis by lung epithelial sphere assay

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Lung epithelial sphere(LES) formation assay seems a good experimental method monitoring lung epithelial stem cells, and sca1-positive cells contain many lung epithelial stem cells. LESs were formed from mouse fetal epithelial cells, which lack sca1-positive cells. The lung epithelial cells having human mutant EGFR gene have high proliferative activity. In these cells, occurrence of sca1-positive cells does no increase, but LEC formation was mildly increased. In addition, significance of stem-cell related molecules was studied using surgically resected samples and cell lines.