The capsule of Staphy lococcus aureus clinical strains

金黄色葡萄球菌临床菌株荚膜

• 批准号: 07670315
• 负责人: UMEDA Akiko
• 金额: $ 0.32万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670315/
• 关键词: Staphylococcus aureus; capsule; pathogenicity; 莱膜

We purified a protein (protein associated with the capsular polysaccharide : PAC) from the capsular layr of clinically isolated encapsulated strains of Staphylococcus aureus. It was a glycoprotein of a molecular size of 200 kDa measured by SDS PAGE.The antiserum raised in a rabbit for PAC reacted not only with the protrein but also with the extracted capsular polysaccharide but not with the cell wall teichoic acid. Immunoelectron microscopic examination of the antiserum treated cell revealed that the antiserum reacted both with the cell wall surface of the encapsulated strain and with the cell wall surface digested with pepsin which removed the cell wall associated proteins almost completely. These results suggested that the sugar moiety of the glycoprotein could be a part of the capsular polysaccharide and the protein could be covalently associated with the capsular layr. The protein had the highly hydrophobic character and it gave the hydrophobic surface character to the encapsulated clinical strains of S.aureus. It can be assumed that the protein may have some roles for the colonization of the bacteria to the host cell surface.

我们从临床分离的金黄色葡萄球菌包囊菌株的荚膜层中纯化了一种蛋白质（与荚膜多糖相关的蛋白质：PAC）。用SDS PAGE测得其分子量约为200 kDa，其抗血清不仅能与蛋白质反应，还能与提取的荚膜多糖反应，但不与胞壁磷壁酸反应。抗血清处理的细胞的免疫电镜检查显示，抗血清与包囊菌株的细胞壁表面和用胃蛋白酶消化的细胞壁表面反应，胃蛋白酶几乎完全除去细胞壁相关蛋白。这些结果表明，糖蛋白的糖部分可能是荚膜多糖的一部分，并且该蛋白可以与荚膜层共价结合。该蛋白具有高度疏水性，使包封的临床菌株具有疏水表面特性。可以假设，该蛋白质可能对细菌在宿主细胞表面的定殖具有某些作用。

项目成果

梅田 昭子: "黄色ブドウ球菌臨床分離株における疎水性莢膜" 臨床と微生物. 23. 358 (1996)

Akiko Umeda：“金黄色葡萄球菌临床分离株的疏水胶囊”《临床和微生物学》23. 358 (1996)。

梅田昭子: "黄色ブドウ球菌臨床分離株における疎水性莢膜" 臨床と微生物. 23. 358- (1996)

Akiko Umeda：“金黄色葡萄球菌临床分离株的疏水胶囊”《临床和微生物学》23. 358- (1996)。