The Caplules of Staphylococcus aureus Clinical Isolates Work as the Colonization Factor

金黄色葡萄球菌临床分离株的荚膜作为定植因子

• 批准号: 09670287
• 负责人: UMEDA Akiko
• 金额: $ 1.98万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670287/
• 关键词: capsule; Staphylococcus aureus; surface hydrophobicity; colonization factor; 萃膜; Staphylococeus aureus; 黄色ブドウ球菌

The rate of encapsulated strains of Stapylococcus aureus is very high among clinical isolates. However, it is still unclear that the capsular polysaccharide of S.aureus has the real pathogenic role as same as other bacterial capsules. Eleven serotypes of capsule are reported and most of clinical isolates belong to the serotype 5 or 8.We observed that the clinical isolates from human blood has the thin layered capsule and the capsular polysaccharide cannnot cover the cell wall teichoic acid. Furthermore, hydrophobic proteins of high molecular weight (200KD and l60KD) strongly bound to the surface of capsule and changed the surface characterisitcs to be hydrophobic. We isolated and purified 200KD protein, one of the hydrophobic proteins, and designated it as CP-PA.The specific antibody against CP-PA reacted on both strains Reynolds and Becker, standard serotype strains of type 5 and type 8 respectively, and it also reacted on many clinical isolated strains. The hydrophobic encapsulated strain NM isolated from human blood highly adhered to PMN cells and Vero cells. These results suggested that the capsular polysaccharide of S.aureus clinical isolates has the pathogenic role as colonization factor to the host cells.

临床分离的金黄色葡萄球菌中有包膜的比例很高。然而，金黄色葡萄球菌的荚膜多糖是否与其他细菌荚膜一样具有真实的致病作用，目前尚不清楚。报道了11种荚膜血清型，临床分离株多为血清5型或8型。观察到人血分离株有薄层荚膜，荚膜多糖不能覆盖胞壁磷壁酸。此外，高分子量的疏水蛋白（200 KD和160 KD）与囊膜表面强烈结合，改变囊膜表面的疏水性。我们分离纯化了一种200 KD的疏水蛋白，命名为CP-PA，其特异性抗体可分别与Reynolds和Becker株、5型和8型标准血清型菌株反应，也可与多种临床分离菌株反应。从人血中分离到的疏水性微囊化菌株NM对PMN细胞和Vero细胞具有较高的粘附性。这些结果表明，金黄色葡萄球菌临床分离株的荚膜多糖作为定植因子对宿主细胞具有致病作用。

项目成果

梅田昭子: "ブドウ球菌の構造と分裂機構" 臨床と微生物. 25・2. 221-222 (1998)

梅田晶子：“葡萄球菌的结构和分裂机制”临床和微生物学25・2（1998）。

Akiko Umeda, Kazunobu Amako: "The hydrophobic capsules of the clinical isolated strains of Staphylococcus aureus." Clinic and Microbiology. Vol.24-2. 220 (1997)

Akiko Umeda、Kazunobu Amako：“临床分离的金黄色葡萄球菌菌株的疏水胶囊。”

梅田昭子・天児和暢: "細菌細胞壁の構造" 日本医真菌学会雑誌. 39・3. 147-150 (1998)

Akiko Umeda 和 Kazunobu Amago：“细菌细胞壁的结构”日本医学真菌学会杂志 39・3（1998）。

Akiko Umeda: "The structure and the division mechanism of Staphylococcus." Clinic and Microbiology. Vol.25-2. 221-222 (1998)

Akiko Umeda：“葡萄球菌的结构和分裂机制。”

梅田 昭子: "ブドウ球菌の構造と分裂機構" 臨床と微生物. 25・2. 221-222 (1998)

梅田明子：“葡萄球菌的结构和分裂机制”临床微生物学25・222（1998）。