Cell cycle regulation of a lympho-hematopoietic specific Rap1GTPase-activating protein, Spa-1 in lymphocyres.

淋巴细胞中淋巴造血特异性 Rap1GTPase 激活蛋白 Spa-1 的细胞周期调节。

• 批准号: 07670367
• 负责人: HATTORI Masakazu
• 金额: $ 1.41万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670367/
• 关键词: Spa-1; Rap1; GAP; cell cycle progression; 11q13.3; Rap1; 11q13.3; リンパ球活性化; 低分子G蛋白; 核内蛋白; プロセッシング; GTPase活性化蛋白; ロイシンジッパー; がん抑制遺伝子

We have cloned a human homologue of mouse Spa-1 gene (SPA-1) which encodes a 130 kDa (p130) cytoplasmic protein with SH3-binding, Rap1GAP.PEST and leucine zipper/coiled coil domains. p130 expressed in a Baculovirus system exhibits a specfic GTPase-activating activity against Rap1. Overexpression of a truncated Spa-1cDNA in NIH3T3 cells apparently disturbed the entry into the cell cycle in the G0-arrested NIH3T3 cells following mitogenic stimulation, suggesting that the protein is critically involved in the cell cycle regulation via the interaction with Rap1. Genomic mapping indicated that Spa-1 is located at the most centromeric region of chromosome 19 in mouse and at the centrimieric neighborhood of CCND1/PRAD1, a presumed BCL1 oncogene, at 11q13.3 in human.

我们克隆了小鼠Spa-1基因（Spa-1）的人同源基因（Spa-1），该基因编码一个130 kDa （p130）的与sh3结合的细胞质蛋白Rap1GAP。PEST和亮氨酸拉链/卷绕线圈领域。杆状病毒系统中表达的p130对Rap1具有特异性的gtpase激活活性。在NIH3T3细胞中，过表达截断的Spa-1cDNA明显干扰了g0阻滞的NIH3T3细胞在有丝分裂刺激后进入细胞周期，这表明该蛋白通过与Rap1的相互作用参与细胞周期调节。基因组图谱显示，Spa-1位于小鼠第19号染色体的着丝粒最集中的区域，在人类中位于假定的BCL1致癌基因CCND1/PRAD1的11q13.3的着丝粒附近。

项目成果

T.Morimura, et al.: "Apoptosis and CD8-down regulation in the thymus of chickens infected wtih Marek's disease virus." Archives of Virology. 141. 2243-2249 (1996)

T.Morimura 等人：“感染马立克氏病病毒的鸡胸腺中的细胞凋亡和 CD8 下调。”

Tosifumi Morimura, et al: "Immuromodulation of periphoral T cells in chickens infected with Morek's disease virus : involvemant in immunosuppression" Journal of General Virology. 76. 2979-2985 (1995)

Tosifumi Morimura 等人：“感染莫里克氏病病毒的鸡周围 T 细胞的免疫调节：参与免疫抑制”普通病毒学杂志。

K.Okada, et al.: "Phenotype analysis of lymphoid cells in Marek's disease of CD4^+ or CD8^+ T cell-.deficient chickens ; Occurrence of double negative T cell tumor." Avian Pathology. (in press).

K.Okada 等人：“CD4+ 或 CD8+ T 细胞缺陷鸡的马立克氏病中淋巴细胞的表型分析；双阴性 T 细胞肿瘤的发生。”

Toshifumi Morimura, et al: "Apoptosis and CD8-down regulation in the thymus of chickens infected with Marek's disease virus." Archiues of Virology. 141. 2243-2249 (1996)

Toshifumi Morimura 等人：“感染马立克氏病病毒的鸡胸腺中的细胞凋亡和 CD8 下调。”

T.Morimura, et al.: "Immunomodulation of peripheral T cells in chickens infected wtih Marek's disease virus : involvement of immunosuppression." Journal of General Virology. 76. 2979-285 (1995)

T.Morimura 等人：“感染马立克氏病病毒的鸡的外周 T 细胞的免疫调节：涉及免疫抑制。”