Effects of Acetaldehyde on Ethanol Absorption in the Rat Jejunum

采用大鼠在体单通道灌流技术，研究了高血乙醛浓度对乙醇在肠道吸收的影响。我们发现，高血乙醛浓度抑制肠道乙醇吸收与乙醛峰值浓度成反比，减少肠道血流量，并抑制肠道2-丁酮吸收。高血乙醛浓度引起的肠道血流量减少是抑制肠道乙醇吸收的主要机制，但其他机制也被认为是抑制吸收的机制。我们观察到在高血乙醛浓度状态下，灌流后收集的液体量增加。由于肠腺分泌增加，我们试图研究灌流后组胺和前列腺素F_2的变化。经三聚氰胺处理后，灌流液中组胺含量增加，而前列腺素F2有升高的趋势。我们不能对各种数据下的结果进行模糊评估，因此，将改为研究灌流后肠道组织中组胺和儿茶酚胺的变化。

We have studied the effects of high blood acetaldehyde concentrations on the intestinal absorption of ethanol using an in situ single-pass perfusion technique on the rat intestine. We found that high blood acetaldehyde concentrations inhibit intestinal ethanol absorption in an inverse proportion to peak acetaldehyde concentrations, decrease intestinal blood flow, and inhibit intestinal absorption of 2-butanone. The decrease of the intestinal blood flow, induced by high blood acetaldehyde concentrations, is a major mechanism inhibiting intestinal ethanol absorption, but other mechanisms are also thought to inhibit absorption.We have observed an increase in volume of collected fluid after perfusionin a state of high blood acetaldehyde concentration. Since secretion from the intestinal glands increases, we tried to study the changes of histamine and prostaglandinF_2 after perfusion. Histamine in perfused fluid increased after pretreatment with cyanamide, but prostaglandinF_2 tended to increase after the pretreatment. We couldn't evaluate the results under various datadue to ambiguity.Therefore, the changes of histamine and catecholamine in intestinal tissue after perfusion will be studied instead.