THE STUDY OF ANGIOGENETIC FACTOR GENE EXPRESSION IN HEPATOCELLULAR CARCINOMA

肝细胞癌血管生成因子基因表达的研究

• 批准号: 07670559
• 负责人: YAMADA Nobuo
• 金额: $ 1.09万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670559/
• 关键词: HEPATOCELLULAR CARCINOMA; ANGIOGENETIC FACTOR; 血管新生因子遺伝子

Northern blot analysis and reverse transcription-polymerase chain reaction (RT-PCR) were used to examine the transcriptional expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and angiogenin in 18 hepatocellular carcinoma (HCC). Correlations between messenger RNA (mRNA) expression and arteriographic findings, as well ashistopathological findings, were studied. Immunohistochemistry was performed to identify the localization of cells expressing VEGF,bFGF,and angiogenin in HCC.Higher levels of VEGF,bFGF,and angiogenin mRNA were observed in 10 cases, 7 cases, 3 cases than in corresponding nontumorous tissues, respectively. The degree of these angiogenetic factor mRNA expression were correlated with the intensity of tumor staining in angiograms, the tumor size, and the histological grade of malignancy. On immunohistochemical obsevation, VEGF and bFGF protein were intensely detected in HCC cells. In contrast, no difference was observed in angiogenin protein. In conclusion, these results suggest that VEGF and bFGF contribute to angiogenesis and tumor growth of HCC.

采用Northern blot和逆转录聚合酶链反应（RT-PCR）技术检测18例肝细胞癌（HCC）组织中血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF）和血管生成素的转录表达。研究了信使RNA （mRNA）表达与动脉造影结果以及组织病理学结果之间的相关性。采用免疫组化方法确定表达VEGF、bFGF和血管生成素的细胞在HCC中的定位。VEGF、bFGF和血管生成素mRNA水平分别高于相应非肿瘤组织10例、7例和3例。这些血管生成因子mRNA的表达程度与血管造影肿瘤染色强度、肿瘤大小及恶性肿瘤的组织学分级有关。免疫组化观察，肝癌细胞中VEGF和bFGF蛋白含量较高。相比之下，血管生成素蛋白没有观察到差异。综上所述，这些结果提示VEGF和bFGF对HCC的血管生成和肿瘤生长有促进作用。