Establishment of universal detection method for virus genome in sera from patients with acute liver damage

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Patients with acute liver damage, who were excluded drug induced, alcholic and autoimmune hepatitis and without serum virus markers of hepatitis A,B and C in acute phase, were studied as subjects in this project. Firstly to deny the existence of hepatitis B virus (HBV) and hepatitis C virus (HCV) in the sera obtained from these patients, nested polymerase chain reaction (PCR) for HBV-DNA at major S region and nested reverse transcription (RT) -PCR for HCV-RNA at 5'-noncoding region were performed. HCV gene was amplified from 4 of the 17 patients and they were diagnosed as acute hepatitis C,however HBV gene was not ampified none of them. Detection of virus genome by these PCR methods was performed in patients with acute hepatitis A and B as controls. It was comfirmed that the timing of sampling and the selection of efficient method for extraction of nucleic acids were important. Secondly to examine the participation of hepatitis G and silent hepatitis B without ordinary HBV markers in the sera from selected patients with acute hepatitis non-A,B,C including 2 fulminant hepatitis, nested RT-PCR for hepatitis G virus (HGV) -RNA at 5'-noncoding region and helicase region and nested PCR for HBV-DNA at precore region were performed. HBV gene was amplified from 8 of the 10 patients, however HGV gene was not ampified none of them. The original plan for the establishment of universal detection method for virus genome in sera from patients with acute liver damage has changed, but it has became clear that the silent HBV infection is the main cause of patients with acute hepatitis non-A,B,C.