The role of cytokines on the production of NO and PGI2 by human pulmonary artery smooth muscle and endothelial cells

细胞因子对人肺动脉平滑肌和内皮细胞产生NO和PGI2的作用

基本信息

  • 批准号:
    07670686
  • 负责人:
  • 金额:
    $ 1.54万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1995
  • 资助国家:
    日本
  • 起止时间:
    1995 至 1996
  • 项目状态:
    已结题

项目摘要

Human pulmonary artery smooth muscle cells (HAPSMC) were cultured from autopsy materials, and the effect of lipopolysaccharide (LPS) and cytokines on PGI_2 production and arachidonic acid metabolism by HPASMC was examined.1) LPS,IL-1_<beta> and TNF_<alpha> enhanced the production of PGI_2 and nitric oxide (NO) by HPASMC.2) HPLC analysis revealed that 10mug/ml of LPS and 200U/ml of IL-1_<beta> enhanced the production of both lipoxygenase (LO) and cyclooxygenase (COX) products, and 500U/ml of TNFalpha enhanced that of COX products.3) IL-6 suppressed the basal production of PGI_2 by HPASMC,and attenuated the enhancement of PGI_2 production by HPASMC treated with LPS,IL-1_<beta> or TNF_<alpha>.4) The enhancement of PGI_2 production by LPS and IL-1_<beta> was attenuated when HPASMC were treated with LPS or IL-1_<beta> together with L-NMMA,NO synthase inhibitor.5) The enhancement of PGI_2 production by LPS and IL-1_<beta> was augmented when HPASMC were treated with LPS or IL-1_<beta> together with sodium nitroprusside, NO donor.6) From these experimental findings, it is suggested that(1) Human pulmonary artery smooth muscle cells could produce NO and PGI_2 in response to LPS or cytokines.(2) NO could enhance the production of PGI_2 by pulmonary artery smooth muscle cells(3) Pulmonary srtery smooth muscles could actively participate in the regulation of pulmonary blood flow and tonus of pulmonary artery by producing NO and PGI_2.Further examination is needed to elucidate the mechanism of the enhancement of PGI_2 production by NO.
采用尸体解剖材料培养人肺动脉平滑肌细胞(HAPSMC),观察脂多糖(LPS)和细胞因子对PGI_2生成和花生四烯酸代谢的影响。1) LPS、IL-1_< β >和TNF_< α >对PGI_2和一氧化氮(NO)的生成均有促进作用。2)HPLC分析表明,10mug/ml LPS和200U/ml IL-1_< β >对脂氧合酶(LO)和环氧合酶(COX)产物的生成均有促进作用,500U/ml TNFalpha对COX产物的生成均有促进作用。3) IL-6抑制了HPASMC产生PGI_2的基础,并减弱了LPS、IL-1_< β >或TNF_< α >对HPASMC产生PGI_2的促进作用。4)LPS、IL-1_< β >联合NO合酶抑制剂L-NMMA对HPASMC产生PGI_2的促进作用减弱。5) LPS或IL-1_< β >联合硝普钠、NO供体对HPASMC细胞PGI_2生成的促进作用增强,IL-1_< β >对PGI_2生成的促进作用增强。6)以上实验结果表明:(1)人肺动脉平滑肌细胞在LPS或细胞因子作用下可产生NO和PGI_2。(2) NO可促进肺动脉平滑肌细胞生成PGI_2。(3)肺动脉平滑肌细胞可通过生成NO和PGI_2,积极参与肺血流和肺动脉张力的调节。NO促进PGI_2生成的机理有待进一步研究。

项目成果

期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Fu-Qiang Wen, Kentaro Watanabe, Minoru Yoshida.: "Eicosanoid profile in cultured human pulmonary artery smooth muscle cells treated with IL-1beta and TNFalpha." Prostaglandins, Leukotrienes and Essential Fatty Acids. (in press).
Fu-Qiang Wen、Kentaro Watanabe、Minoru Yoshida.:“用 IL-1beta 和 TNFalpha 处理的培养人肺动脉平滑肌细胞中的类二十烷酸谱。”
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  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Fu-Qiang Wen,Kentaro Watanabe,Minoru Yoshida.: "Inhibitory effect of Interleukin-6 on release of PGI_2 by cultured human pulmonary artery smooth muscle cells." Prostaglandins. 52. 93-102 (1996)
Fu-Qiang Wen,Kentaro Watanabe,Minoru Yoshida.:“白细胞介素6对培养的人肺动脉平滑肌细胞释放PGI_2的抑制作用。”
  • DOI:
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    0
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Fu-Qiang Wen,Kentaro Watanabe Hideki Tanaka,Minoru Yoshida.: "Cytokines and lipopolysaccharide enhance basal and thrombin-stimulated production of PGI_2 by cultured human pulmonary artery smooth muscle cells." Prostaglandins,Leukotrienes and Essential Fat
Fu-Qiang Wen、Kentaro Watanabe、Hideki Tanaka、Minoru Yoshida.:“细胞因子和脂多糖增强培养的人肺动脉平滑肌细胞的基础和凝血酶刺激的 PGI_2 产生。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Fu-Qiang Wen,Kentaro Watanabe,Minoru Yoshida.: "Eicosanoid profile in cultured human pulmonary artery smooth muscle cells treated with IL-1β and TNFα." Prostaglandins, Leukotrienes and Essential Fatty Acids. (印刷中).
Fu-Qiang Wen、Kentaro Watanabe、Minoru Yoshida:“用 IL-1β 和 TNFα 处理的培养人肺动脉平滑肌细胞中的类二十烷酸谱。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Fu-Qiang Wen,Kentaro Watanabe Minoru Yoshida.: "Eicosanoid profile in cultured human pulmonary artery smooth muscle cells treated with IL-1β and TNFα." Prostaglandins,Leukotrienes and Essential Fatty Acids. (印刷中).
Fu-Qiang Wen、Kentaro Watanabe Minoru Yoshida:“用 IL-1β 和 TNFα 处理的培养人肺动脉平滑肌细胞中的类二十烷酸谱。”
  • DOI:
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  • 影响因子:
    0
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WATANABE Kentaro其他文献

WATANABE Kentaro的其他文献

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{{ truncateString('WATANABE Kentaro', 18)}}的其他基金

Development of Work Knowledge Circulation Method for Service Fields
服务领域工作知识流通方法开发
  • 批准号:
    15K16174
  • 财政年份:
    2015
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Development of a Technology Selection Method of Process Observation and Modeling for Labor-Intensive Services
劳动密集型服务过程观测与建模技术选择方法的开发
  • 批准号:
    25730190
  • 财政年份:
    2013
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Development of cathodoluminescence nanospectroscopy for the insulating nanostructures
绝缘纳米结构阴极发光纳米光谱学的发展
  • 批准号:
    23760022
  • 财政年份:
    2011
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
THE DIAGNOSIS AND THE TREATMENT BY STEROIDS IN DIOPATHIC INTERSTITIAL PNEUMONIAS BASED ON THE TRANSBRONHIAL LUNG BIOPSY
基于经支气管肺活检的特发性间质性肺炎的诊断和类固醇治疗
  • 批准号:
    16590770
  • 财政年份:
    2004
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Meso-pelagic animal behavior study by animal borne camera and 3-D loggers
利用动物相机和 3D 记录仪进行中上层动物行为研究
  • 批准号:
    14405027
  • 财政年份:
    2002
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The role of nitric oxide in PGI_2 production by human pulmonary artery smooth muscle cells
一氧化氮在人肺动脉平滑肌细胞产生PGI_2中的作用
  • 批准号:
    09670636
  • 财政年份:
    1997
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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