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Role of mast cells in allergic inflammation in the skin

肥大细胞在皮肤过敏性炎症中的作用

基本信息

批准号：
07670930
负责人：
KUROSAWA Motohiro
金额：
$ 1.41万
依托单位：
Gunma Uinversity
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670930/
关键词：
allergy mast cell tryptase chymase substance P ultraviolet light mastocytosis mediator メディアエーター全身性肥満細胞症 IgE

项目摘要

Mast cells were raised from umbilical cord blood cells in the presence of stem cell factor and interleukin 6. Immunocytochemical staining for mast cell proteases revealed that all cells contained tryptase. Anti-IgE effectively stimulated the mast cells to release histamine in a dose-dependent, time-dependent manner. Calcium ionophore A23187 also induced histamine release in a dose-dependent, time-dependent manner. Compund 48/80 and substance P failed to induce histamine release from the cells. As a next step of the study, the effects of ultraviolet light irradiation on substance P-induced histamine release from rat peritoneal mast cells were investigated. Ultraviolet light B (UVB) irradiation inhibited the histamine release in a dose-dependent manner, and 8-methoxypsoralen plus long-wave ultraviolet light (UVA) irradiation inhibited in a dose-dependent manner of UVA.UVB irradiation and 8-methoxypsoralen plus UVA irradiation inhibited the histamine release through the suppression of intracellular calcium concentrations.Mast cell subtype of patients with mastocytosis was investigated. Mast cells containing tryptase and chymase were the only type seen in the skin lesions of a patient with urticaria pigmentosa. Mast cells containing tryptase and chymase were predominant in the nonlesional skin of a patient with indolent systemic mastocytosis, however mast cells containingtryptase (but not chymase) were predominant in the lesional skin of the patient. Biopsy specimens from not only the skin but also the lymphnode, bone marrow, bronchus, nasal mucosa, gastric mucosa showed infiltrations of mast cells containing tryptase (but not chymase) in a patient with systemic mastocytosis. Blood levels of histamine, tryptase, leukotriene C4 and urine levels of methylhistamine, leukotriene E4 were elevated in the patients with mastocytosis.

在干细胞因子和白细胞介素6的存在下，从脐带血细胞中培养肥大细胞。肥大细胞蛋白酶的免疫细胞化学染色显示，所有的细胞含有类胰蛋白酶。抗IgE抗体能有效刺激肥大细胞释放组胺，并呈剂量依赖性、时间依赖性。钙离子载体A23187也以剂量依赖、时间依赖的方式诱导组胺释放。化合物48/80和P物质不能诱导细胞释放组胺。作为研究的下一步，紫外线照射对P物质诱导的大鼠腹腔肥大细胞组胺释放的影响进行了研究。紫外线B（UVB）照射可剂量依赖性地抑制组胺的释放，8-甲氧基补骨脂素（8-methoxypropylene）加长波紫外线（UVA）照射可剂量依赖性地抑制UVA的释放，UVB照射和8-甲氧基补骨脂素加UVA照射可通过抑制细胞内钙离子浓度来抑制组胺的释放。肥大细胞含有类胰蛋白酶和糜蛋白酶是唯一的类型看到在皮肤病变的患者色素性荨麻疹。肥大细胞主要含有类胰蛋白酶和糜蛋白酶的非病变皮肤的惰性全身肥大细胞增多症患者，然而肥大细胞含有类胰蛋白酶（但不糜蛋白酶）的患者的病变皮肤中占主导地位。一例全身性肥大细胞增多症患者的皮肤活检标本以及淋巴结、骨髓、支气管、鼻粘膜、胃粘膜活检标本均显示含有类胰蛋白酶（但不含糜蛋白酶）的肥大细胞浸润。肥大细胞增多症患者血组胺、类胰蛋白酶、白三烯C4及尿甲基组胺、白三烯E4均升高。