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Peptide Histidine Isoleucine (PHI) in the Respiratory Tract

呼吸道中的肽组氨酸异亮氨酸 (PHI)

基本信息

批准号：
02670336
负责人：
KUROSAWA Motohiro
金额：
$ 0.51万
依托单位：
Gunma University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670336/
关键词：
PHI VIP immunoreactivity respiratory tract airway resistance superoxide anion inflammatory cells leukotriene C_4 rat guinea pig 非アドレナリン非コリン作動性抑制神経神経ペプチド

项目摘要

(1) A high titer of peptide histidine isoleucine (PHI) antiserum which did not react with vasoactive intestinal peptide (VIP) was obtained from a rabbit immunized with rat PHI and rat PHI radioimmunoassay was established with this antiserum. Tracheas, extrapulmonary bronchi and lungs were dissected from rats sacrificed by microwave irradiation. They were homoginized with 0.5 N acetic acid and centrifuged. Lyophilized supernates were used as samples for the radioimmunoassay. PHI immunoreactivity occurred more in the central airways than in the peripheral ones. VIP immunoreactivity in rat respiratory tracts, assayed simultaneously by radioimmunoassay using commercialized VIPantiserum, was as strong as that of PHI, suggesting that PHI and VIP may be produced from the same precursor at the ratio of 1:1 in rat respiratory tracts.(2) Intravenous administration of VIP or PHI reduced dynamic respiratory resistance; however, the effect was significantly less than that of isoprenaline. VIP and P … More HI inhibited the increase in dynamic respiratory resistance by intravenous administration of histamine in a dose-dependent manner, suggesting that VIP and PHI may be involved in the pathogenesis of airway hyperresponsiveness through inhibiting the effect of mediator release on the airway.(3) VIP inhibited superoxide anion production in a dose- dependent manner by the activated peripheral blood neutrophils, mononuclear cells and also by the human monoblast cell line U937, the capacity of which for superoxide anion formation was induced by the pretreatment of interferon-gamma. 3x10^<-6> M VIP also inhibited superoxide anion generation by the activated peripheral blood eosinophils and alveolar macrophages obtained by bronchoalveolar lavage, suggesting VIP may serve as an endogenous modulator of inflammatory reactions in the respiratory tract.(4) A high titer of PHI antiserum which did not react with VIP was obtained from a rabbit immunized with rat PHI and PHI like immunoreactivity in the respiratory tract from guinea pigs was measured by radioimmunoassay. Tracheas, extrapulmonary bronchi and lungs were dissected and treated by microwave irradiation. They were homogenized with 0.5 N acetic acid and centrifuged. Lyophilized supernates were used as samples for the radioimmunoassay. PHI like immunoreactivity was present more in the central airways than in the peripheral ones. Intravenous administration of leukotriene C_4 reduced significantly PHI like immunoreactivity in the tracheas and the extrapulmonary bronchi in parallel with the significant increase of dynamic respiratory resistance. Less

(1)用大鼠组氨酸异亮氨酸（PHI）免疫家兔，获得了与血管活性肠肽（VIP）不发生反应的高效价肽类PHI抗血清，并以此建立了大鼠PHI放射免疫分析法。微波照射后处死大鼠，解剖气管、肺外支气管和肺。用0.5 N乙酸匀浆并离心。冻干上清液用作放射免疫测定的样品。中心气道的PHI免疫反应性高于外周气道。用放射免疫法同时测定VIP在大鼠呼吸道的免疫反应性，结果表明VIP与PHI的免疫反应性相当，提示在大鼠呼吸道中PHI和VIP可能是由同一前体以1：1的比例产生的。(2)静脉注射VIP或PHI可降低动态呼吸阻力，但其作用明显小于异丙肾上腺素。VIP和P ...更多信息 HI可剂量依赖性地抑制组胺引起的动态呼吸阻力的增加，提示VIP和PHI可能通过抑制气道介质释放而参与气道高反应性的发生。(3)VIP以剂量依赖性方式抑制活化的外周血中性粒细胞、单核细胞以及人单核细胞系U937的超氧阴离子产生，所述人单核细胞系U937的超氧阴离子形成能力由干扰素-γ预处理诱导。3 × 104 μ <-6>M VIP还可抑制通过支气管肺泡灌洗获得的活化外周血嗜酸性粒细胞和肺泡巨噬细胞产生超氧阴离子，表明VIP可作为呼吸道炎症反应的内源性调节剂。(4)用大鼠PHI免疫家兔，获得高滴度的不与VIP反应的PHI抗血清，用放射免疫法测定豚鼠呼吸道中的PHI样免疫反应性。解剖气管、肺外支气管和肺，并进行微波照射。用0.5 N乙酸匀浆并离心。冻干上清液用作放射免疫测定的样品。PHI样免疫反应在中央气道较周围气道多。静脉注射白三烯C_4可显著降低气管和肺外支气管的PHI样免疫反应性，同时使动态呼吸阻力显著增加。少