歯痛受容機構の免疫学的・電気生理学的研究

牙痛受体机制的免疫学和电生理学研究

• 批准号: 07771732
• 负责人: 池田 英治
• 金额: $ 0.77万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07771732/
• 关键词: odontoblast(象牙芽細胞); in-vitro immunization; モノクローナル抗体; patch-clamp法

これまでの研究で明らかになったことは以下の3点である.まず第一に,歯髄細胞(主に象牙芽細胞)を生活性を失わずに単離することに成功した.これにより抗体産製のための抗原を高密度で確保することができ,かつ電気生理学的なアプローチが可能になった.第二に,In vitro immunization法を用いて、象牙芽細胞と樹状歯髄細胞を同定できるモノクローナル抗体産生に成功した.幾千もの産製抗体から4種類を選択した.すなわち(1)象牙芽細胞の細胞質とミエリン鞘を染めるIgG抗体,(2)象牙芽細胞のplasmamembraneを特異的に染めるIgG抗体,(3)象牙芽細胞の核と細胞質および象牙芽細胞層下層の細胞の核を染めるIgG抗体,(4)象牙芽細胞,dendritic cell,血管内側のendothelial cellを染めるIgM抗体である.これらを独自にあるいは組み合わせて使用することにより、cryostat標本上でも単離細胞においても象牙芽細胞とその下層の樹枝状細胞の同定が可能になった.第三に,電気生理学的にpatch-clamp法を応用して電極先端と象牙芽細胞膜間にギガオームレベルの高抵抗シールに成功し,single channel記録に成功した.歯科領域でのin vitro immunization法と象牙芽細胞へのpatch-clamp法応用は世界初の試みであり、この研究は歯髄細胞機能を解明する出発点となりうると考えられる。

Please do some research on the following three o'clock points. In the first place, the cells of the ivory buds (the main ivory buds) lost their viability and became successful. Antibodies are used to detect antigens and high-density antigens to ensure that they are not susceptible to radioactivity, while those in the physiology of electrical machines may be affected. In the second step, the In vitro immunization method was used to determine the antibody production successfully. Select one of the four types of antibodies. The ivory bud cells were stained with IgG antibodies, (2) the ivory bud cells were stained with plasmamembrane specific IgG antibodies, (3) the ivory bud cells were stained with IgG antibodies, (4) the ivory bud cells were stained with IgM antibodies, (4) the ivory bud cells were stained with IgM antibodies. The target cells are isolated from the cryostat tag, the ivory germ cells are isolated from the ivory germ cells, the dendritic cells are the same as that of the dendritic cells. In the third chapter, the patch-clamp method of computer physiology was used to use the front end of the electrodes, the ivory germ intermembrane chromatography, the high resistance test, the success test and the single channel record success test. In the field of science and technology, in vitro immunization method, ivory germ cell method, patch-clamp method was used to understand the location of ivory germ cells in the world.

项目成果

H.Ikeda et al.: "Monoclonal antibodies raised to cat odontoblasts by in vitro immunization" Quint.(in the press). (1996)

H.Ikeda 等人：“通过体外免疫产生猫成牙本质细胞的单克隆抗体”Quint.（媒体）。

H.Ikeda et al.: "Immuno-labelling of pulpal nerves and odontoblasts in cat teeth" J.dent.Res. 74. 404

H.Ikeda 等人：“猫牙中牙髓神经和成牙本质细胞的免疫标记”J.dent.Res。

H.Ikeda,et al.: "Importance of clinical examination and diagnosis A case dens invaginatus" Oral Surg. 79. 88-91 (1995)

H.Ikeda 等：“临床检查和诊断的重要性一例牙内陷”口腔外科。

H.Ikeda: "The isolation of odontoblasts from cat teeth" J.Physiol.476. 12-13 (1994)

H.Ikeda：“从猫牙中分离成牙本质细胞”J.Physiol.476。

H.Ikeda,et al.: "Three groups of offerent pulpal feline nerve fibres show different electrophysiological response properties" Archs Oral Biol.40. 895-904 (1995)

H.Ikeda 等人：“三组猫科动物牙髓神经纤维表现出不同的电生理反应特性”Archs Oral Biol.40。