咀嚼圧が義歯床下上皮組織の活性動態に及ぼす影響に関する研究

咀嚼压力对义齿基托下上皮组织活动动力学影响的研究

• 批准号: 07771844
• 负责人: 原 哲也
• 金额: $ 0.64万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07771844/
• 关键词: 咀嚼圧; 義歯床下粘膜上皮組織; 上皮細胞活性; AgNOR染色; ラット

本研究はラットの臼歯部口蓋を対象として,義歯床を介して加えられる咀嚼圧によって惹起される義歯床下粘膜上皮細胞の活性動態の変化について,上皮基底細胞核が示すNORs(nucleolar organizer regions)数を指標として,上皮組織に惹起される病理組織学的変化との関連において検討することを目的とした.15週齢のウイスター系雄性ラット120匹(義歯装着群3群と対照群1群 各30匹)を用いた.義歯装着群に対しては,安静時には義歯床下組織と無圧の状態で接触し,咬合時には規定量(100,50および13μm)だけで沈下する義歯床を装着した.対照群は義歯を装着することなく経過させた.義歯床装着1,2,4,8,12および20週後に各群の5匹ずつを屠殺して義歯床下の口蓋粘膜を採取し,4%パラホルムアルデヒドを用いて固定した後,通法に従ってパラフィン包埋した.2μmのAgNOR染色標本を対象として,各実験条件毎に上皮基底細胞核1個当たりのNORs数の平均値を求め,各実験群間の有意差検定を行った.また,4μmのヘマトキシリン-エオジン染色標本を対象として上皮組織の病理組織学的変化を観察した.その結果,義歯床下粘膜の上皮基底細胞核の示したNORs数は,病理組織学的変化との関連を示して変化した.義歯床下粘膜上皮に退行性の組織変化がみられた義歯床装着1および2週後のNORs数は,いずれの義歯装着群においても対照群に比べて有意に減少すると同時に,義歯床の沈下量の増加に伴って減少した.4週後の義歯装着群のNORs数はいずれも,対照群に比べれば有意に低い値を示すものの,上皮組織の増殖性変化に伴って2週後に比べて増加して各沈下群ともほぼ同様の値を示した.8週後以降は,13μm沈下群のNORs数が経時的にわずかに増加して対照群と同様の値を示したが,50および100μm沈下群では対照群ならびに13μm沈下群に比べて,その差は大きくはないものの,有意に低い値を示した.

In this study, the mouth of the molars and molars in the molars showed that the number of nors (nucleolar organizer regions) in the basal nucleus of the epithelium indicated that the number of nors (nors) in the basal nucleus of the epithelium indicated that the number of nors (nors) in the basal nucleus of the epithelium indicated that the number of nors (nors) in the basal nucleus of the epithelium indicated that the number of nors (nors) in the nucleus of the basal epithelium indicated that the number of nors (nors) in the basal nucleus of the epithelium indicated that the number of nors (nors) in the nucleus of the basal epithelium indicated that the number of nors (nors) The epithelial tissue caused the histology of histopathology. The purpose of this study was to induce the histology of the epithelium. the purpose of this study was to induce the histology of epithelial tissue. The purpose of this study was to induce the histology of epithelial tissue. The purpose of this study was to induce the histology of epithelial tissue. The purpose of this study was to induce the histology of epithelium. there were 120 males (30 males each). The machine is equipped with a mass machine, the tissue under the bed is free of contact when it is quiet, and there is no contact with it during the occlusal period (100 minutes 50 minutes 13 μ m). In the light of the group, they pretended to pass by. The bed is equipped with 1pm, 2m, 8pm, 12h, 20s, each group, 5 horses, each group, each group, the mucous membrane of the mouth under the bed, the mouth mucosa, the mucous membrane, the epithelium, the number of NORs, the number of cells, and the number of cells. There is an intentional difference between different groups. In this study, we examined the histology of epithelial tissue and histopathology of epithelial tissue. The results showed that the basal nucleus of the subbedded mucosa showed the number of NORs, and the histology of histology showed histological changes. The degenerative tissue of the mucosal epithelium under the bed is equipped with the number of NORs after 1 year and 2 years, and the number of photos is less than that of the intended group. At the same time, the volume of the bed is less than that of the intended one. 4 years later, there is a cluster of NORs data, which is shown to be lower than the intended value. The epithelial tissue metastasization is associated with the growth of the epithelia. After 2 weeks, each submerged group shows the same value. 8 days later, 13 μ m, 13 μ m, 50 μ m, 100 μ m, 50 μ m, 100 μ m, 13 μ m, 50 μ m, 100 μ m, 50 μ m, 100 μ m, 13 μ m, 13 μ m, 50 μ m, 100 μ m, 13 μ m, 50 μ m, 100 μ m, 13 μ m, 13 It is intentional to show that it is low.