Phylogenetic relationship of Galliform subfamilies by hemoglobin

通过形态分类、dna杂交、溶菌酶c氨基酸序列和细胞色素b核酸序列数据分析加利形亚科的系统发育关系。然而，这些结果显示了不同的系统发育树。我们利用Hb氨基酸序列数据探讨了胆杆菌亚科的系统发育关系。在第一个方案中，它考虑到禽类血液的获取困难，计划使用PCR，幸运的是，可以获得加州鹌鹑和几内亚家禽的血液。认为我们得到了一些蒜型香料的氨基酸序列数据。我们测定了加利福尼亚鹌鹑和珍珠鸡的血红蛋白氨基酸序列。在Galliforms的部分香料的氨基酸序列数据中，增加了两个新的数据，分别用最大似然法和邻接法进行了分析。因此，从血红蛋白数据中得到的系统发育树与从线粒体DNA数据和DNA杂交中得到的树相似。这两棵树颠倒了加州鹌鹑和几内亚家禽之间的关系。在这些研究的同时，我们开发了针对难取血液的稀有物种的PCR应用程序。我们改进了一种从羽毛中提取DNA的新方法，该方法比传统的方法提取的DNA得率更高。最近，我们从鸡毛中提取了DNA，通过聚合酶链式反应可以扩增出珠蛋白基因。

Phylogenetic relationships of Galliform subfamilies analyzed by morphological classification, DNA hybridization, Lysozyme c amino acid sequence data and cytochrome b nucleic acid sequence data. However, these results were showed the different phylogenetic trees. We approach phylogenetic relationship of subfamilies of Galliforms by hemoglobin amino acid sequence data. In the first plan, it considered that the acquirement of the avian blood was difficult, it planned to use PCR.The blood of California quail and Guinea fowls could be luckily, acquired. It considered that we got the amino acid sequence data of some spices of Galliforms. We determined the hemoglobin amino acid sequence of California quail and Guinea fowls. The amino acid sequence data of some spices of Galliforms, added two new data of California quail and Guinea fowls, analyzed by maximum likelihood method and neighbor joining method. As a result, phylogenetic tree from hemoglobin data resembled tree from the mitochondria DNA data and DNA hybridization. These two trees were reversed the relationship between California quail and Guinea fowls. In parallel of these study, we developed the PCR application for the rare species which difficult to get the blood. We improved a new method of DNA extraction from the feather which got high yield as the conventional method. Recently, we extracted DNA from the chicken feather and the PCR reaction can amplify of globin gene.