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Phylogenetic relationship of Galliform subfamilies by hemoglobin

通过血红蛋白分析鸡形亚科的系统发育关系

基本信息

批准号：
07804061
负责人：
EGUCHI Yukinori
金额：
$ 1.41万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07804061/
关键词：
Hemoglobin Amino Acid Sequence Molecular Evolution Phylogenetic Tree キジ亜科キジ科

项目摘要

Phylogenetic relationships of Galliform subfamilies analyzed by morphological classification, DNA hybridization, Lysozyme c amino acid sequence data and cytochrome b nucleic acid sequence data. However, these results were showed the different phylogenetic trees. We approach phylogenetic relationship of subfamilies of Galliforms by hemoglobin amino acid sequence data. In the first plan, it considered that the acquirement of the avian blood was difficult, it planned to use PCR.The blood of California quail and Guinea fowls could be luckily, acquired. It considered that we got the amino acid sequence data of some spices of Galliforms. We determined the hemoglobin amino acid sequence of California quail and Guinea fowls. The amino acid sequence data of some spices of Galliforms, added two new data of California quail and Guinea fowls, analyzed by maximum likelihood method and neighbor joining method. As a result, phylogenetic tree from hemoglobin data resembled tree from the mitochondria DNA data and DNA hybridization. These two trees were reversed the relationship between California quail and Guinea fowls. In parallel of these study, we developed the PCR application for the rare species which difficult to get the blood. We improved a new method of DNA extraction from the feather which got high yield as the conventional method. Recently, we extracted DNA from the chicken feather and the PCR reaction can amplify of globin gene.

通过形态分类、DNA杂交、溶菌酶c氨基酸序列和细胞色素B核酸序列分析鸡形亚科的系统发育关系。然而，这些结果显示了不同的系统发育树。利用血红蛋白氨基酸序列资料探讨鸡形目各亚科的系统发育关系。第一种方案考虑到禽类血液获取困难，拟采用PCR技术，幸运地获得了加州鹌鹑和几内亚鸡的血液。认为本研究获得了鸡形目部分香料的氨基酸序列数据。我们测定了加州鹌鹑和几内亚鸡血红蛋白的氨基酸序列。鸡形目部分香料的氨基酸序列数据，增加了加州鹌鹑和珍珠鸡的两个新数据，采用最大似然法和近邻连接法进行分析。结果表明，血红蛋白数据的系统发育树与线粒体DNA数据和DNA杂交的系统发育树相似。这两棵树颠倒了加州鹌鹑和几内亚鸡之间的关系。在这些研究的同时，我们开发了PCR应用于稀有物种，难以获得血液。我们改进了一种从羽毛中提取DNA的新方法，该方法与传统方法相比，获得了较高的提取率。近年来，我们从鸡的羽毛中提取DNA，通过PCR反应可以扩增出珠蛋白基因。