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Search of new polyisoprenoid-related compounds

寻找新的聚异戊二烯相关化合物

基本信息

批准号：
07806018
负责人：
ISHINAGA Masataka
金额：
$ 1.09万
依托单位：
Hiroshima Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07806018/
关键词：
Dolichol Polyisoprenoid Prenol Aging Rat Dietary Protein ポリプレノール脂質脂質過酸化反応酵母加令

项目摘要

This glant was Grant-in-Aid for Exploratory Research. I could not identify new polyisoprenoid-related compounds this time. In the near future, I will identify the new compounds.Search of new polyisoprenoid-related compounds Inspection of the reversed HPLC pattern of the neutral lipid fraction of rat spleen reveals, in addition to dolichol and dolichy fatty acid esters, the presence of not vet identified compounds in the most apolar lipid region. The unknown compounds consisted on esterified dolichol (C_<85>-C_<100>), and did not fatty acids. Amounts of these compounds increased with aging, and with diet containing casein.Effects of aging and diet on the accumulation of dolichol in rat tissues. A diet containing 15% (W/W) fat and 20% (W/W) of either casein or soy protein was fed to rats for 18 months. The contents of spleen dolichols in rats fed the soy protein diet tended to be higher than those in rats fed the casein diet. The amounts of dolichols in heart and brain were not influenced by the diet. The proportion of spleen dolichyl fatty ester in rats fed the experimental diets was higher than that in rats fed the lab chow. The distribution of the dolichol isoprenologues was not influenced by the diet. There was a shift in the dolichol isoprenologues in kidney and spleen toward ones of lower chain length until 2 months of age, and after that there was no change. However, in heart and brain they shifted toward ones of lower chain length with aging.There are a lot of amounts of polyprenol in rat adipose tissue Usually, the alpha-unsaturated from (polyprenol) of polyisoprenoid occurs in small amounts (less than 1% of total polyisoprenoid). I found for the first time that polyprenol of the adipose tissue of around spleen was about 60% of total polyisoprenoid in rat.

这笔赠款是探索性研究资助金。这次我无法识别新的聚异戊二烯相关化合物。在不久的将来，我将鉴定新的化合物。搜索新的聚异戊二烯相关化合物对大鼠脾脏中性脂质部分的反向 HPLC 模式的检查表明，除了多甘醇和多甘醇脂肪酸酯外，在最非极性的脂质区域中还存在未经兽医鉴定的化合物。未知化合物由酯化多甘醇(C_ 85 -C_ 100 )组成，而不是脂肪酸。这些化合物的含量随着衰老和含有酪蛋白的饮食而增加。衰老和饮食对大鼠组织中多萜醇积累的影响。给大鼠喂食含有 15% (W/W) 脂肪和 20% (W/W) 酪蛋白或大豆蛋白的饮食 18 个月。饲喂大豆蛋白饲料的大鼠脾脏多羟基化合物的含量往往高于饲喂酪蛋白饲料的大鼠。心脏和大脑中多羟基化合物的含量不受饮食的影响。饲喂实验饮食的大鼠中脾脏多甘醇脂肪酸酯的比例高于饲喂实验室饲料的大鼠。多醇异戊二烯同体的分布不受饮食影响。直到 2 个月大时，肾脏和脾脏中的多甘醇异戊二烯同系物才发生向链长度较短的转变，此后就没有变化。然而，在心脏和大脑中，随着年龄的增长，它们转向较短链长的链长。大鼠脂肪组织中存在大量聚异戊二烯醇。通常，聚异戊二烯的α-不饱和（聚异戊二烯醇）含量很少（小于总聚异戊二烯的 1%）。我首次发现大鼠脾脏周围脂肪组织中的聚异戊二烯含量约占总聚异戊二烯的60%。