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A study of the properties of oligotrophic bacteria

寡营养细菌特性的研究

基本信息

批准号：
07807033
负责人：
TADA Yoshifumi
金额：
$ 1.34万
依托单位：
KINKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07807033/
关键词：
Oligotrophic bacteria Membrane protein two-dimenshional electropholysis 膜構成蛋白質

项目摘要

Membrane proteins of oligotrophic bacteria were analyzed by two -dimensional electropholysis. Oligortrophic bacteria could grow even in extremely poor medium, diluted nutrient broth, that was diluted with distillled water (1/10,000). However, they also could grow in rich medium. We speculated that had ability to uptake nutrition outside efficiently through membrane when they grow in poor medium. They may change the membrane proteins composition for efficient uptake of nutrition in poor medium. Then we analyzed membrane proteins isolated from the same bacteria growing in rich medium and poor medium respectively. Membrane proteins of three species of oligotrophic bacteria were investigated. They were all identified as Pseudomonas cepacia.Considerable differences in two-dimensional electropholysis patterns of membrane proteins were found between growing conditions. Especially in P.cepacia I that could grow in poor medium weel, during the transition from rich medium to poor medium total elctrophlysis pattern changed fairly as if they had been different strains. In spite of the decrease of the total number of proteins, several new proteins of rich amount appeared in membrane of bacteria growing in poor medium. The change of the pattern was increasing as nutrition in medium was removed. Especially, as the nutrition was diluted to 1/100, the pattern was changed considerably. In other strains, total elctropholysis patterns were not changed and new proteins were not found, but the concentration of several membrane proteins increased in poor medium. These results suggest that oligotrophic bacteria produce some membrane proteins much more to make transport channel for efficientl uptake of nutrition.

利用双向电泳技术对贫营养菌的膜蛋白进行了分析.寡营养细菌甚至可以在用盐水稀释（1/10，000）的极差培养基、稀释的营养肉汤中生长。但是，它们也可以在丰富的培养基中生长。我们推测，当它们生长在不良培养基中时，能够通过膜有效地吸收外界营养。它们可以改变膜蛋白的组成，以便在贫培养基中有效地吸收营养。然后，我们分析了从同一细菌分别在富培养基和贫培养基中生长的膜蛋白。对3种贫营养细菌的膜蛋白进行了研究。结果表明，不同生长条件下，细菌膜蛋白的二维电泳图谱存在较大差异。特别是洋葱P.cepacia I，在贫培养基中生长良好，从富培养基向贫培养基过渡时，总电解质模式发生了很大变化，就像是不同的菌株。在贫培养基中生长的细菌，其细胞膜上的蛋白质总量虽有所减少，但仍出现了一些新的蛋白质，且含量丰富。随着培养基中营养物质的去除，这种变化趋势逐渐增强。特别是当营养液稀释到1/100时，格局发生了很大变化。在其他菌株中，总的电解模式没有改变，没有发现新的蛋白质，但几个膜蛋白的浓度增加，在贫培养基。这些结果表明，贫营养细菌产生更多的膜蛋白，为营养物质的有效吸收提供运输通道。