Studies for pathogenesis of erythema migrans caused by Lyme disease Borrelia

莱姆病疏螺旋体引起的游走性红斑发病机制的研究

• 批准号: 07807032
• 负责人: ISOGAI Emiko
• 金额: $ 1.34万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07807032/
• 关键词: Lyme disease; Borrelia burgdorferi; erythema migrans; platelet-activating-factor; inflammatory cytokines; platelets; neutrophils; endothelial cells; Borrelia Burgdorferi; 游走性紅斑; Borrilia burgdorferi; 血小板活性化因子(PAF)

This study describes a role of platelet-activating factor (PAF) as a potential inducer of inflammation in infection with Borrelia burgdorferi. Following approaches were done.1) The first involved the use of a PAF antagonist to show the lack of an inflammatory response in skin lesions.2) The second was to show that the PAF antagonist reduced platelet aggregation when the spirochetes were incubated with polymorphonuclear leukocytes (PMN).3) The third was to show that the PMN,which were activated by the spirochetes, inhibited collagen-platelet aggregation.3) The forth was to show the effect of spirochetes on the adherence of platelets and PMN to cultured human umbilical vein endothelial cells (HUVEC). The PAF antagonist reduced the cell-adherence to HUVEC.These observations may contribute to a better underestanding of the pathogenesis of Lyme disease spirochetes in the local inflammatory reaction.

这项研究描述了血小板活化因子（PAF）作为一个潜在的诱导炎症感染伯氏疏螺旋体的作用。第一种方法是使用PAF拮抗剂来显示皮肤病变中缺乏炎症反应。第二种方法是显示当螺旋体与多形核白细胞（PMN）孵育时PAF拮抗剂减少血小板聚集。第三种方法是显示被螺旋体激活的PMN，（3）螺旋体对血小板和中性粒细胞（PMN）与培养的人脐静脉内皮细胞（HUVEC）粘附的影响。PAF拮抗剂可降低细胞与HUVEC的粘附性，这一结果有助于更好地了解莱姆病螺旋体在局部炎症反应中的发病机制。

项目成果

Isogai, E., Isogai, H., Kimura, K., Hayashi, S., Kubota, T., Nishikawa, T., Nakane, A., and Fujii, N.: "Cytokines in the serum and brain in mice infected with distinct species of Lyme disease Borrelia." Microbial Pathogenesis. 21. 413-419 (1996)

Isogai, E.、Isogai, H.、Kimura, K.、Hayashi, S.、Kubota, T.、Nishikawa, T.、Nakane, A. 和 Fujii, N.：“小鼠血清和大脑中的细胞因子

磯貝恵美子 他: "我が国のライム病の微生物学的特徴" 獣医畜産新報. 48. 588-591 (1995)

Emiko Isogai 等人：“日本莱姆病的微生物特征”《兽医和牲畜报纸》48. 588-591 (1995)。

Kimura,K.,Isogai,E.et al.: "Prevalence of antibodies against Borrella species in patients with unclassified uveitis in regions in with Lyme disease is endemic" Clin.Diag.Labo.Immunol.2. 53-56 (1995)

Kimura,K.,Isogai,E.等人：“莱姆病地区未分类葡萄膜炎患者中抗疏螺旋体抗体的流行是地方性的”Clin.Diag.Labo.Immunol.2。

Yukikake,M.,Kuroda,Y.et al.: "Occurrence of Borrelia garinii oculo-neuroborreliosis in Japan." J.Neuro.Sci.142. 160-161 (1996)

Yukikake,M.,Kuroda,Y.et al.：“Borrelia garinii 眼神经疏螺旋体病在日本的发生。”

Ishii, N., Isogai, E., Isogai, H., Kimura, K., Nishikawa, T., Fujii, N., and Nakajima, H.: "T cell response to Borrelia garinii, Borrelia afzelii, and Borrelia japonica in various congenic mouse strains." Microbiol.Immunol.39. 929-935 (1995)

Ishii, N.、Isogai, E.、Isogai, H.、Kimura, K.、Nishikawa, T.、Fujii, N. 和 Nakajima, H.：“T 细胞对加氏疏螺旋体、阿氏疏螺旋体和日本疏螺旋体的反应