Structure-related activity of tick defensin and antibody monitoring system in wildlife

野生动物中蜱防御素和抗体监测系统的结构相关活性

• 批准号: 22590381
• 负责人: ISOGAI Emiko
• 金额: $ 2.25万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590381/
• 关键词: マダニ; デフェンシン; 野生動物; モニタリング; ライム病; 抗菌ペプチド; 立体構造; 抗体; 生態系; エゾシカ; 自然免疫; 共生

Defensins are major group of antimicrobial peptides and are found widely in vertebrates, invertebrates and plants. Invertebrate defensins have been identified in ticks. In this study, chemically synthesized tick defensins were used to investigate the activity spectrum against bacteria isolated from the midgut. Synthetic tick defensin showedantimicrobial activity against Staphylococcus aureus but not Borrelia garinii and symbiotic Stenotrophomonas maltophila. The results demonstrate that the activity of antimicrobial peptides from tick is important for selection and reservation of specific bacteria such as Lyme disease spirochetes in the midgut. The teriary structure brought more potent activity to S. aureus than primary one in short-time killing. The killing activity is related to the structure of tick defensing. Antibody against tick defensing in wildlife monitoring system can useful to know the risk of tick-borne diseases.

防御素是一类主要的抗菌肽，广泛存在于脊椎动物、无脊椎动物和植物中。无脊椎动物防御素已经在扁虱中被发现。在这项研究中，使用化学合成的扁虱防御素来研究其对从中肠分离的细菌的活性谱。合成的扁虱防御素对金黄色葡萄球菌显示出抗菌活性，而对加里氏疏螺旋体和共生的麦芽窄食单胞菌没有显示出抗菌活性。结果表明，硬蜱抗菌肽的活性对筛选和保存莱姆病螺旋体等特定细菌具有重要意义。在短时杀灭中，三叉树结构对金黄色葡萄球菌的活性比初级结构更强。其杀灭活性与壁虱的防御结构有关。在野生动物监测系统中，抗蚤防御抗体有助于了解蜱传疾病的风险。

项目成果

Analysis of genetic background in disease-sensitivity associatedwith Lyme disease

莱姆病相关疾病敏感性遗传背景分析

• DOI: 10.5924/abgri.39.26
• 发表时间: 2011
• 期刊: The Journal of Animal Genetics
• 作者: 磯貝恵美子;米山裕;磯貝浩
• 通讯作者: 磯貝浩

犬のライム病、動物の感染症(第3版)

犬莱姆病，动物传染病（第三版）

• 发表时间: 2011
• 作者: Isogai E;Isogai N;Sato K Yoneyama H;Fukuda T;Deguchi Y;Matsubara K;Murata H;Tuboi T;Haritani M;Miyamoto T;Isogai H;磯貝恵美子;磯貝恵美子;磯貝恵美子(分担)
• 通讯作者: 磯貝恵美子(分担)

Correlation between levels of antibody against Lyme disease Borrelia and tick defensin in wild deer.

野鹿莱姆病疏螺旋体抗体和蜱防御素水平之间的相关性。

• 发表时间: 2012
• 作者: Isogai H;Isogai E.
• 通讯作者: Isogai E.

Wild deer have antibodies recognizing tick defensin with tertiary structure, Joint Meeting of the 17th International Symposium on Gnotobiology (ISG) and the 34th Congress of Society for Microbial Ecology and Disease (SOMED)

野鹿具有识别三级结构蜱防御素的抗体，第十七届国际昆虫生物学研讨会（ISG）和第34届微生物生态与疾病学会（SOMED）联席会议

• 发表时间: 2011
• 作者: Isogai E;Isogai N;Sato K;Yoneyama H;Fukuda T;Deguchi Y;Matsubara K;Murata H;Tuboi T;Haritani M;Miyamoto T;Isogai H
• 通讯作者: Isogai H

Homology modeling and tertiary structure-related activity of tick defensin (persulcatusin) in the taiga tick

针叶林蜱中蜱防御素（persulcatusin）的同源建模和三级结构相关活性

• 发表时间: 2011
• 作者: Isogai E;Saito T;Ohmura T;Isogai H;Okumura K;Hori H;Yoneyama H;Tsuruta H;Kurebayashi Y.
• 通讯作者: Kurebayashi Y.