Synthesis and Structure-Function Relationship Study of Pore Structures emulated Ligand-gated Ionic Channel

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(1)A peptide with the predicted transmembrane sequence of botulinum neurot6xin A was synthesized. Single-channel recording data shows that it forms cation-selective ionic channels in lipid bilayers.(2)A few template-assembled helix bundle proteins emulated the pore structure of acetyicholine receptor were synthesized by a new chemoselective-ligation method developed by us.(3)Ion permeability of a membrane with soft polar interfaces with fixed charges and dipoles were investigated theoretically.(4)A peptide with the predicted transmembrane sequence of M2 proteins of influenza virus A was synthesized, and its structure in bilayer membranes and function of proton transport were studied.(5)Effect of co-ions on proton conductance of gramicidin A channel were investigated by the single channel recording method, and results were analyzed by a theoretical model.(6)Stability and structure of non-bilayer structures of biomembranes such as hexagonal II and interdigitated gel phases were studied by X-ray diffraction and fluorescence spectroscopy. These results indicate that interaction free energy between segments of membrane surface and solvents plays an important role in the stability of these membranes.(7)Polymorphism of F-actin assembly was studied, and its quantitative phase diagram was made.