Study on interactions of proteins/peptides disrupting cell membranes with lipid membranes using the single giant unilamellar vesicle method

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We investigated the interactions of proteins/peptides disrupting cell membranes with lipid membranes using the single GUV method, where GUV is giant unilamellar vesicle with diameter greater than 10μm. We found that the rate constants of antimicrobial peptide magainin 2-induced pore formation in lipid membranes are mainly determined by the surface concentration of magainin 2 in the membranes. The measurement of the rate constants of membrane permeation of fluorescent probes through the magainin 2-induced pores revealed that the radius of the pore changes with time at the pore formation. We also revealed the pore formation induced by protein toxin lysenin, which can bind sphingomyelin specifically, and obtained membrane permeability coefficients of a fluorescent probe, calcein, through the pores in various lipid membranes