TREATMENT OF METASTATIC LIVER TUMORS BY INTERMITTENT REPETITIVE INJECTION OF A ANGIOGENESIS INHIBITOR USING AN IMPLANTABLE PORT SYSTEM

使用植入式端口系统间歇性重复注射血管生成抑制剂治疗转移性肝肿瘤

• 批准号: 07671002
• 负责人: HIROTA Shozo
• 金额: $ 1.6万
• 依托单位: KOBE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671002/
• 关键词: angiogenesis inhibitor; TNP 470; liver metastasis; implantable port; intermittent injection; intraportal injection; rabbit; 門脈内間欠反復注入; ウサギ(家兎); 悪性腫瘍; 門脈内注入; 抗癌剤

TNP-470 is expected as a new type of anticancer drug which prevents tumor neovascularization by acting as angiogenesis inhibitor. We attempted to evaluate anticancer effect of intermittent repetitive intraportal injection of TNP-470 for metastatic hepatic tumor models using VX2-carcinoma and considered usefulness and role of TNP-470.We used 25 Japanese white rabbits with metastatic hepatic tumors made by intraportal injection of tumor cells, and divided into five groups ; group A was control group, group B was treated by repetitive subcutaneous injection of TNP-470 and group C,D,E was treated by repetitive intraportal injection of TNP-470, adriamycin, TNP-470+adriamycin respectively 5 times at 2 or 3 days interval after tumor inoculation using portal reservoir systems. After 2 weeks, we examined anticancer effect of TNP-470 pathologically.In the number of grown tumors, there was no remarkable difference among five groups statisitically. But mean tumor size of each group is smaller in order of group E,D,C,B,A and the differences were significant (P<0.05) except between E and D group. And intratumoral neovascularization tended to be less in group B,C,E than in group A,D pathologically.We consider that intermittent repetitive injection of TNP-470 via reservoir system is more useful than systemic administration for liver metastasis.

TNP-470有望成为一种新型抗癌药物，通过充当血管生成抑制剂来防止肿瘤新生血管形成。我们尝试评估间歇性重复门静脉内注射TNP-470对使用VX2-癌的转移性肝肿瘤模型的抗癌效果，并考虑TNP-470的有用性和作用。我们使用25只通过门静脉内注射肿瘤细胞制成的转移性肝肿瘤日本白兔，分为五组； A组为对照组，B组采用TNP-470重复皮下注射，C、D、E组采用门静脉储库系统接种肿瘤后，分别每隔2、3 d门静脉内重复注射TNP-470、阿霉素、TNP-470+阿霉素5次。 2周后，我们通过病理学检查TNP-470的抗癌效果。在生长肿瘤的数量方面，五组之间没有统计学上的显着差异。但各组平均肿瘤大小依次为E、D、C、B、A组较小，且除E、D组间差异均有统计学意义（P<0.05）。病理上B、C、E组瘤内新生血管较A、D组少。我们认为通过储库系统间歇性重复注射TNP-470比全身给药对肝转移更有效。