A Trial Of Differentiating Hepatocellular Carcinoma From Analogous Lesions In Rat Experimental Tumor Models Using Iron Colloid Enhanced MR Imaging.

为探讨铁胶体增强磁共振成像(MRI)在鉴别肝细胞癌(HCC)和增生性结节病变(HN)中的作用，用~(59)Fe标记的硫酸软骨素胶体(CSIC)对大鼠肿瘤模型进行显微放射自显影。将20只大鼠随机分为3组，即正常组、肝癌组和HN组。肝细胞癌组给予0.06%3‘-二甲基二胺盐酸盐(DAB)诱导肿瘤，连续3个月。HN组间歇给药0.025%乙酰氨基荧组，连续4个月。将肝细胞癌模型分为两种类型：高分化肝细胞癌(HCC-W)、中至低分化肝细胞癌(HCC-MP)。通过显微放射自显影进行定量分析，计算每视野内Kupffer细胞的数量。此外，暗视野上的图像被传输到图像处理系统。计算光敏面积比(PAR)。在HN和正常肝组织中，Kupffer细胞的数量和PAR均无显著差异。在肝细胞癌-W细胞中，Kupffer细胞也存在。Kupffer细胞的数量和PAR明显低于正常肝组织。在HCC-MP中，Kupffer细胞也呈散在分布。Kupffer细胞数和PAR均显著低于HCC-W组。结论：铁胶体增强MRI对鉴别肝细胞癌与肝细胞癌及诊断肝细胞癌的分化程度有一定的价值。

To demonstrate the usefulness of iron colloid-enhanced MR images (MRI) in differentiating between hepatocellular carcinoma (HCC) and hyperplastic nodular lesions (HN), microautoradiograms were prepared after administration of chondroitin sulfate iron colloid (CSIC) labelled with 59Fe in rat tumor models. Twenty rats were divided into 3 groups, normal, HCC and HN groups. In the HCC,0.06% 3'-Mmethydiaminobenzine tetrahydro-chloride (DAB) was administered for 3 consecutive months for tumor induction. In the HN,0.025% acetylaminofluorene (AAF) was intermittently administered for 4 months. HCC models were divided into 2 types, well-differentiated HCC (HCC-W), moderately to poorly-differentiated HCC (HCC-MP). By quantitative analysis using microautoradiography, the number of Kupffer cells per field of view was calculated. Furthermore, the images on the dark fields were transferred to an image processing system. A photosensitized area ratio (PAR) was calculated. These ratios were compared among groups.There was no significant difference in either the number of Kupffer cells or PAR between HN and normal liver tissues. In HCC-W,Kupffer cells were also present. However, their distribution was scattered, and the number of Kupffer cells and the PAR were significantly lower than those in normal liver tissues. In HCC-MP,the distribution of Kupffer cells was also scattered. Both the number of Kupffer cells and the PAR were significantly lower than those in HCC-W.In conclusion, iron colloid-enhanced MRI is useful for differentiating HCC from HN and diagnosing the degree of differentiation of HCC.