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A Trial Of Differentiating Hepatocellular Carcinoma From Analogous Lesions In Rat Experimental Tumor Models Using Iron Colloid Enhanced MR Imaging.

使用铁胶体增强 MR 成像在大鼠实验肿瘤模型中区分肝细胞癌和类似病变的试验。

基本信息

批准号：
07671004
负责人：
SUTO Yuji
金额：
$ 1.54万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671004/
关键词：
MRI Liver Contrast Agent Iron collid Animal Autoradiography Cancer

项目摘要

To demonstrate the usefulness of iron colloid-enhanced MR images (MRI) in differentiating between hepatocellular carcinoma (HCC) and hyperplastic nodular lesions (HN), microautoradiograms were prepared after administration of chondroitin sulfate iron colloid (CSIC) labelled with 59Fe in rat tumor models. Twenty rats were divided into 3 groups, normal, HCC and HN groups. In the HCC,0.06% 3'-Mmethydiaminobenzine tetrahydro-chloride (DAB) was administered for 3 consecutive months for tumor induction. In the HN,0.025% acetylaminofluorene (AAF) was intermittently administered for 4 months. HCC models were divided into 2 types, well-differentiated HCC (HCC-W), moderately to poorly-differentiated HCC (HCC-MP). By quantitative analysis using microautoradiography, the number of Kupffer cells per field of view was calculated. Furthermore, the images on the dark fields were transferred to an image processing system. A photosensitized area ratio (PAR) was calculated. These ratios were compared among groups.There was no significant difference in either the number of Kupffer cells or PAR between HN and normal liver tissues. In HCC-W,Kupffer cells were also present. However, their distribution was scattered, and the number of Kupffer cells and the PAR were significantly lower than those in normal liver tissues. In HCC-MP,the distribution of Kupffer cells was also scattered. Both the number of Kupffer cells and the PAR were significantly lower than those in HCC-W.In conclusion, iron colloid-enhanced MRI is useful for differentiating HCC from HN and diagnosing the degree of differentiation of HCC.

为了证明铁胶体增强磁共振成像（MRI）在区分肝细胞癌（HCC）和增生性结节性病变（HN）中的有用性，在大鼠肿瘤模型中给予59 Fe标记的硫酸软骨素铁胶体（CSIC）后制备了显微放射自显影图。将20只大鼠分为正常组、肝癌组和HN组。在HCC中，连续3个月给予0.06%3 '-甲基二氨基苯四氢氯化物（DAB）诱导肿瘤。HN组间断给予0.025%乙酰氨基芴（AAF）4个月。HCC模型分为高分化HCC（HCC-W）和中分化至低分化HCC（HCC-MP）两种类型。通过使用显微放射自显影术的定量分析，计算每个视野的枯否细胞数量。此外，将暗场上的图像转移到图像处理系统。计算光敏面积比（PAR）。各组间比较，HN与正常肝组织间枯否细胞数及PAR均无显著性差异。在HCC-W中，还存在枯否细胞。但其分布较分散，枯否细胞数量和PAR均明显低于正常肝组织。在HCC-MP中，枯否细胞也呈散在分布。Kupffer细胞数量和PAR均显著低于HCC-W。结论：铁胶增强MRI有助于HCC与HN的鉴别及HCC分化程度的诊断。