ApolipoproteinE gene polymorphism in frontotemporal dementia in Japan

日本额颞叶痴呆的载脂蛋白E基因多态性

• 批准号: 07671074
• 负责人: OGOMORI Koji
• 金额: $ 1.47万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671074/
• 关键词: dementia; apolipoproteinE; molecular biology; frontotemporal dementia; Pick's desease; 前頭-側頭型痴呆

Introduction : Many previous reports have shown a relationship between the apolipoprotein E (ApoE) epsilon 4 allele and an increased risk of developing Alzheimer's disease (AD). Some studies have also reported a relationship between ApoE epsilon 4 allele and frontotemporal dementia (FTD), while others have failed to show any such association. The relationship between the ApoE epsilon 4 allele and FTD thus remains controversial. As aresult, we analyzed the ApoE genotype in patients with FTD in Japan.Subjects and methods : We analyzed the ApoE genotypes in 30 patients with FTD,35 patients with AD and 31 normal subjects using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) according to the protocol of Hixson et al. and Wenham et al. The diagnosis was made clinically according to the NINCDS-ADRDA criteria for AD and the Lund and Manchester Groups criteria for FTD.We determined the allelic frequencies for each group by counting the alleles and calculating the sample proportions. Group differences in the allelic frequencies were analyzed by the chi^2 test.Results : The frequencies of the ApoE epsilon 4 allele in FTD,AD and the control were 7/60 (11.7%), 23/70 (32.8%) and 2-62 (3.2%), respectively. The chi^2 test showed no significant difference in the ApoE epsilon 4 allele frequency between FTD and the control (p=0.0746), while that the same test did show a significant difference in the ApoE epsilon 4 allele frequency between FTD and AD (p=0.0070).Conclusion : These results indicate that no association apparently exists between the ApoE epsilon 4 allele and FTD in Japan.

介绍：许多先前的报告已经显示载脂蛋白E（ApoE）104等位基因与发展阿尔茨海默病（AD）的风险增加之间的关系。一些研究也报道了ApoE ε 4等位基因与额颞叶痴呆（FTD）之间的关系，而另一些研究则未能显示任何此类关联。因此，ApoE β 4等位基因与FTD之间的关系仍存在争议。因此，我们分析了日本FTD患者的ApoE基因型。我们分析了30例FTD患者的ApoE基因型，采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）技术，对35例AD患者和31例正常人进行了检测，并与希克森等和温哈姆等的方法进行了比较。AD采用ADRDA标准，FTD采用隆德和曼彻斯特组标准，通过计数等位基因和计算样本比例确定各组的等位基因频率。结果：FTD组ApoE α 4等位基因频率为7/60（11.7%），AD组为23/70（32.8%），对照组为2-62（3.2%）。卡方检验显示FTD和对照组之间的ApoE ε 4等位基因频率无显著差异（p=0.0746），而同一检验显示FTD和AD之间的ApoE ε 4等位基因频率存在显著差异（p=0.0070）。结论：这些结果表明ApoE ε 4等位基因与日本FTD之间不存在明显相关性。