Heat shock proteins in animal models for acute renal failure and in human discased kidneys

热休克蛋白（Heat shock protein，HSPs）是细胞对各种应激反应的一种快速合成蛋白。这些蛋白质在进化过程中高度保守。最近，我们已经纯化了哺乳动物HSP 60、HSP 73和HSP 90，并制备了针对这些HSP的特异性抗体。我们先前报道了药物介导的急性肾功能衰竭动物模型中HSP73和HSP90的诱导和改变的定位。在本报告中，我们已经研究了进一步的诱导和改变本地化的热休克蛋白在其他动物模型急性肾损伤和人类患病的肾脏。1：在大鼠肾脏短暂缺血后，热休克蛋白73和热休克蛋白90诱导近端小管损伤和再生上皮细胞的细胞质。肾提取物的系列免疫印迹分析显示，在缺血后的退行性和再生阶段的HSP 73和HSP 90的双相诱导。这些结果提示热休克蛋白在靶细胞的保护和修复中具有重要作用 关于我们 肾缺血。2：在庆大霉素致急性肾小管损伤的大鼠肾组织中，光镜水平的免疫组化研究表明，HSP 73在损伤的近曲小管上皮细胞的溶酶体中被诱导和积聚。我们已经证实了这一点，使用电子显微镜免疫组织化学。HSP73可能在庆大霉素肾病靶细胞修复受损蛋白的处置中发挥作用。3：在正常大鼠肾脏中，HSP60定位于肾小管上皮细胞的细胞质和线粒体中。此外，HSP 60在Henle袢和集合管之间的肾小管上皮细胞的腔侧表达。限水后，在肾小管上皮细胞的腔侧的HSP 60的诱导大于在正常肾脏中观察到的。肾乳头提取物的免疫印迹分析，发现与水限制的HSP 60的量增加。提示HSP 60可能在水重吸收过程中起作用。4.在人病肾中，HSP 73和HSP 90均在肾小球新月体和再生肾小管细胞中被诱导表达。这些结果表明，热休克蛋白在细胞新月体的形成和损伤细胞的修复中具有重要作用。少

Heat shock proteins (HSPs) are rapidly synthesized in cells in response to a varicty of stresses. These proteins are highly conserved through evolution. Recently, we have purified mammalian HSP60, HSP73, and HSP90, and produced specific antibodies against these HSPs. We previously reported the induction and altered localization of HSP73 and HSP90 in animal models for drug-mediated acute renal failure. In the present report, we have examined further the induction and altered localization of HSPs in other animal models for acute renal injury and in human diseased kidneys.1 : In rat kidneys after transient ischemia, both HSP73 and HSP90 were induced in the cytoplasm of injured and regenerative epithelial cells of proximal tubules. Serial immunoblot analysis of renal extracts revealed the biphasic induction of HSP73 and HSP90 during the degenerative and regenerative phases after ischemia. These results may indicate that HSPs have important roles in the protection and repair of target cells … More from renal ischemia.2 : In rat kidneys with gentamicin-induced acute tubular injury, immunohistochemiocal studies at light microscopic level showed that HSP73 appcaredto be induced and accumulated in the lysosomes of damaged epithelial cells of proximal tubules. We have confirmed this using electron microscopic immunohistochemistry. HSP73 may have a role in the disposition of damaged proteins for repair of target cells from gentamicin nephropathy.3 : In normal rat kidneys, HSP60 was localized within the cytoplasm and the mitochondria in tubular epithelial cells. In addition, HSP60 was expressed at the luminal side of tubular epithelial cells between the Henle's loops and collecting ducts. After water-restriction, the induction of HSP60 at the luminal side of tubular epithelial cells was greater than that observed in normal kidneys. On immunoblot analysis of renal papilla extracts, increased amounts of HSP60 were found in association with water-restriction. These results suggest that HSP60 may play a role in water reabsorption.4.In human diseased kidneys, both HSP73 and HSP90 were induced in the cellular crescent of the glomeruli and regenerative tubular cells. These results suggest that HSPs have important roles both in the formation of cellular crescents and in the repair of injured cells. Less