Induction of drug induced tolerance in mice and application for large animals

从1995年进行的分析中，得到以下结果：1)嵌合现象仅在淋巴细胞水平发生，未在骨髓水平发生。2) B10D2和B10 （MHC单独分离）结合时，克隆破坏，嵌合诱导是短暂的；3)移植皮存活时间延长与嵌合状态持续时间有显著关系。从1996年开始，我们开始分析我们的新方案（SC+CP+Busulfan+骨髓细胞方案），以突破MHC障碍。我们得到了非常令人鼓舞的数据，在传统的CP诱导耐受方案下，使用丁硫凡观察到永久嵌合和植皮存活。我们想要达到的最终目标是仅通过药物在大型动物中诱导耐受性。我们认为我们的新数据将会取得更大的成功。

From analysis performed in 1995, the following results were obtained. 1) Chimerism was induced only in lymphocytes, and wasn't induced in bone marrow level. 2) In the combination of B10D2 and B10 (MHC alone disparate), clonal destruction was observed, but the induction of chimerism was transient, and 3) skin graft survival prolongation and duration of chimeric state had significnat relationship.From 1996, we started the analysis of our new protochol (SC+CP+Busulfan+Bone marrow cells protochol) to break through the MHC obstacle. We got very encouraging data that permanent chimerism and skingraft survaival were observed by using busulfan with our traditional CP induced tolorance protochol.The final goal that we want to achive is to induce tolerance only by drugs in large animals. And we think our new data is going to be a greater success.