Prevention of hyperacute xenograft rejection by polyclonal antibodies
多克隆抗体预防超急性异种移植排斥反应
基本信息
- 批准号:07671339
- 负责人:
- 金额:$ 1.47万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Prevention of hyperacute xenograft rejection by polyclonal antibodies against donor luymphocytes and red cells.T.Murakami, S.Hujita, Y.Hayasaka, M.Shinkai, Y.Funakoshi, T.Kawai, S.Fuchinoue, S.Teraoka, T.Agishi, and K.Ota.Dept.of Surgery, Kidney Center, Tokyo Womens Medical College, Tokyo, Japan.Intravenous administration of immunoglobulin (IVIG) at a high dosage has been reported to delay an initiation of hyperacute rejection (HAR) of the discordant xeno-grafts. In order to investigate possibility of preventing HAR of guinea pig hearts grafted to Lewis rats by IVIG,specific polyclonal antibodies were produced by immunizing rabbits against guinea pig lymphocytes and red cells (anti-GP-pAb)Immediately before transplantation, the Lewis rats intravenously received either 1 ml of 20% human albumin as a control or 1.5-30mg/kg of the antibodies. Contraction time of the vascualized cardiac xenografts was extended from a mean of 12.2 minutes to longer than 7 hours intimately depending upon the doses of the antibodies administered (r=0.958). Immunopathological studing showed diffuse deposition of C3 and fibrinogen with a small amount of vascular deposits of rat IgM in the rejected grafts. In the functioning grafts treated with the antibodies, rabbit IgG deposition along endothelium was observed early after transplantation and becam more extensive with toime, while deposits of C3, fibrinogen and rat IgM were minimal. In conclusion, our data suggest that the rabbit antiGP-pAb. at a high dosage prevent the binding of the natural antibodies to the graft endothelium and modulate the complement activation.
通过抗供体淋巴细胞和红细胞的多克隆抗体预防超急性异种移植排斥反应。T.Murakami,S.Hujita,Y.Hayasaka,M.Shinkai,Y.Funakoshi,T.Kawai,S.Fuchinoue,S.Teraoka,T.Agishi和K.Ota。东京女子医学院肾脏中心外科,东京,据报道,静脉注射高剂量的免疫球蛋白(IVIG)可延迟超急性排斥反应(HAR)的发生。异种移植物的不协调性为探讨静脉注射丙种球蛋白(IVIG)预防刘易斯大鼠心脏移植后HAR的可能性,在移植前即刻用豚鼠淋巴细胞和红细胞免疫家兔,制备特异性多克隆抗体(抗GP-pAb)。刘易斯大鼠静脉注射20%人血白蛋白1 ml或1.5 ~ 30 mg/kg。血管化的心脏异种移植物的收缩时间从平均12.2分钟延长至超过7小时,这与所施用的抗体的剂量密切相关(r=0.958)。免疫病理学研究显示排斥移植物中弥漫性C3和纤维蛋白原沉积,以及少量大鼠IgM血管沉积。在用抗体处理的功能性移植物中,在移植后早期观察到兔IgG沿着内皮沉积沿着,并且用托伊梅变得更广泛,而C3、纤维蛋白原和大鼠IgM的沉积最小。结论:兔抗GP-pAb.在高剂量下防止天然抗体与移植物内皮的结合并调节补体激活。
项目成果
期刊论文数量(13)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
藤田省吾: "抗異種赤血球/白血球ポリクローナル抗体による生着延長効果" 日本移植学会雑誌(総会臨時号). 30. 137 (1995)
Shogo Fujita:“抗异种红细胞/白细胞多克隆抗体延长植入的效果”日本移植学会杂志(大会特刊)30. 137(1995)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Touru Murakami: "prevention of hyperacute Xenograft rejection by polyclonal antibodies" INFA and WAITS 1995 in Sappro Abs.99 (1995)
Touru Murakami:“通过多克隆抗体预防超急性异种移植排斥”INFA 和 WAITS 1995 in Sappro Abs.99 (1995)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
早坂勇太郎,他: "Long Term-DSTによる抗ドナー異種既存(赤血球凝集)抗体の抑制に関する検討" 日本移植学会雑誌『移植』(総会臨時号). Vol.30. 309- (1995)
Yutaro Hayasaka等:“长期-DST抑制抗供体异种预先存在(红细胞凝集)抗体的研究”日本移植学会杂志“移植”(大会特刊)卷。 . 30. 309- (1995)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Y.Hayasaka, et al.: "Prevention of anti-donor Xenopreformed antibodies with Long Term-DST" 「Transplantation」 Japanese Transplantation Association.
Y.Hayasaka 等人:“用长期 DST 预防抗供体异种预制抗体”“移植”日本移植协会。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
S.Fujita,et al.: "Prevention of hyperacute xenograft rejection by polyclonal antibodies against donor blood cells." Transplantation Proceedong. 28(2). 726 (1996)
S.Fujita 等人:“通过抗供体血细胞的多克隆抗体预防超急性异种移植排斥。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
HAYASAKA Yutaro其他文献
HAYASAKA Yutaro的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
相似国自然基金
新城疫病毒重组异源基因疫苗特异性抗肿瘤研究
- 批准号:81172139
- 批准年份:2011
- 资助金额:65.0 万元
- 项目类别:面上项目
Endoglin适配体靶向异源基因疫苗抗肿瘤研究
- 批准号:81072161
- 批准年份:2010
- 资助金额:34.0 万元
- 项目类别:面上项目
超急性排斥反应靶向抗肿瘤自杀基因疫苗的研究
- 批准号:30660185
- 批准年份:2006
- 资助金额:24.0 万元
- 项目类别:地区科学基金项目
相似海外基金
Immune response and viral dynamics within the female genital tract during hyperacute and acute HIV infection
超急性和急性艾滋病毒感染期间女性生殖道内的免疫反应和病毒动态
- 批准号:
10547981 - 财政年份:2022
- 资助金额:
$ 1.47万 - 项目类别:
Immune response and viral dynamics within the female genital tract during hyperacute and acute HIV infection
超急性和急性艾滋病毒感染期间女性生殖道内的免疫反应和病毒动态
- 批准号:
10667441 - 财政年份:2022
- 资助金额:
$ 1.47万 - 项目类别:
Elucidation of the mechanism by which NRDC, a multifunctional protease, regulates platelet production and its significance as a diagnostic marker for ACS in the hyperacute phase.
阐明多功能蛋白酶 NRDC 调节血小板生成的机制及其作为超急性期 ACS 诊断标志物的意义。
- 批准号:
20K08445 - 财政年份:2020
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Lipid-conjugated Heteroduplex oligonucleotide administration for ischemic stroke in the hyperacute phase
脂质缀合异源双链寡核苷酸给药治疗超急性期缺血性中风
- 批准号:
20K07733 - 财政年份:2020
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Active Learning Tool Development for Promoting Resilience in Hyperacute Disaster Nursing
开发主动学习工具以提高超急性灾难护理的复原力
- 批准号:
16H07117 - 财政年份:2016
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Research Activity Start-up
Development of novel cancer immunotherapy utilizing hyperacute rejection induced by alpha-gal epitope
利用α-gal表位诱导的超急性排斥反应开发新型癌症免疫疗法
- 批准号:
26560447 - 财政年份:2014
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Architectural Re-planning on Medical Electronic Devices Management Section, Operating Department and NICU for Use and Management of Transportable Medical Electronic Devices in Hyperacute Hospitals
超急症医院移动式医疗电子设备使用和管理的医疗电子设备管理科、手术部和NICU架构重新规划
- 批准号:
24560755 - 财政年份:2012
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Attenuation of hyperacute dysfunction and coagulopathy in GalT-KO pulmonary xenotransplantation
GalT-KO 肺异种移植中超急性功能障碍和凝血病的减轻
- 批准号:
23390337 - 财政年份:2011
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Probing retinal and neural mechanisms of vision by hyperacute optical stimulation of single photoreceptor cells
通过单个感光细胞的超急性光刺激探索视觉的视网膜和神经机制
- 批准号:
190271452 - 财政年份:2010
- 资助金额:
$ 1.47万 - 项目类别:
Research Fellowships
Change in blood pressure in hyperacute stroke patients evaluated using ambulatory blood pressure monitoring
使用动态血压监测评估超急性卒中患者的血压变化
- 批准号:
20591039 - 财政年份:2008
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)